CELLULAR AND MOLECULAR BIOLOGY OF LIPOPROTEIN METABOLISM

此应用程序代表计划项目的延续，其中 总的主题是研究脂蛋白与细胞和 纸巾。这项研究的重点主要集中在：1)角色 载脂蛋白在决定脂蛋白识别和命运中的作用，2) 蛋白质和脂肪随后运动的动力学和机制 细胞和脂蛋白之间的关系；3)细胞的储存和代谢 脂类。这些研究的目标是提供关于以下方面的基本信息 涉及脂类和脂蛋白代谢的机制 将促进我们对导致形成和形成的事件的理解 动脉粥样硬化斑块消退。此应用程序由以下部分组成 由三个核心实验室支持的四个密切相关的项目：1) 行政/中央核心，2)组织培养核心，以及3)脂蛋白 核心。项目1提出了关于合成和代谢的研究。 脂蛋白，特别是载脂蛋白B。 将使用从整体动物到亚细胞的实验系统 分数。项目2包括将侧重于这一运动的研究。 培养中细胞间脂质和脂蛋白的相互作用，以及 脂蛋白在这一运动中的酶促修饰。这些研究 将利用培养的细胞、亚细胞部分和各种 包括酶、脂蛋白和脂类在内的分子。项目3建议 将阐明一些结构-功能关系的研究 脂蛋白；尤其值得关注的是胆固醇的作用机制 运动和脂类和蛋白质之间的相互作用 脂蛋白。在这个项目中，建议的实验将使用 各种实验方法，从培养细胞到 用物理方法观察的单个分子的相互作用。项目 4详细介绍了一些实验，这些实验将解决 脂蛋白，特别是乳糜粒，与细胞和组织，与 强调视黄醇和视黄酸酯的运输。实验系统 在这个项目中，范围从整个动物到In 酶和脂类分子的体外研究。一组调查人员 组成该计划的项目在LID方面有相似的兴趣和目标 和脂蛋白代谢，同时提供广泛的科学专业知识。这个 这些研究人员涵盖的科学学科包括 生物化学、生理学、物理化学、细胞和分子生物学 和营养。

This application represents a continuation of a Program Project with an overall theme to study the interaction of lipoproteins with cells and tissues. The major emphasis of this research focuses on I) the role of apoproteins in determining the recognition and fate of lipoproteins, 2) the kinetics and mechanisms of any consequent movement of proteins and lipid between cells and lipoproteins, and 3) the cellular storage and metabolism of lipids. The goal of these studies is to provide basic information on the mechanisms involved in the metabolism of lipids and lipoproteins which will advance our understanding of the events leading to the formation and regression of the atherosclerotic plaque. This application consists of four closely related projects supported by three Core Laboratories: 1) Administrative/Central Core, 2) Tissue Culture Core, and 3) Lipoprotein Core. Project 1 proposes studies on the synthesis and metabolism of lipoproteins with particular emphasis on apoprotein B. The investigations will use experimental systems ranging from whole animals to subcellular fraction. Project 2 consists of studies which will focus on the movement of lipid between cells in culture and lipoproteins, and the influence of enzymatic modification of lipoproteins on this movement. These studies will utilize cultured cells, subcellular fractions and a variety of molecules including enzymes, lipoproteins and lipids. Project 3 proposes studies that will elucidate some structure-function relationships in lipoproteins; of particular concern are the mechanisms of cholesterol movement and the interaction between lipids and proteins within lipoproteins. In this project, experiments are proposed that will use a variety of experimental approaches ranging from cultured cells to the interaction of individual molecules as viewed by physical methods. Project 4 details a number of experiments which will address the interaction of lipoproteins, particularly chylomicrons, with cells and tissue, with emphasis on transport of retinol and retinyl esters. Experimental systems in this Project range over the entire spectrum from whole animals to In Vitro studies on enzymes and lipid molecules. The group of investigators comprising this Program Project share similar interests and goals in lipid and lipoprotein metabolism while providing broad scientific expertise. The scientific disciplines encompassed by these investigators include biochemistry, physiology, physical chemistry, cell and molecular biology and nutrition.