NEUROMUSCULAR AND CENTRAL NERVOUS SYSTEM DISEASES AND THEIR EXPERIMENTAL MODELS

在肌肉和神经活检中进行酶组织化学 用于几种神经肌肉疾病患者的诊断目的 紊乱 免疫细胞化学研究采用 胸腺肽的特异性抗体，以研究 胸腺上皮细胞的分布 重症肌无力 淋巴细胞之间的相互作用 和中枢神经系统进行了研究， 它们细胞表面的共同抗原标记 胸腺素β 4， 免疫调节多肽，被发现是一种常见的 巨噬细胞、树突状淋巴细胞和 少突胶质细胞 某些患者的IgM 副蛋白血症性多发性神经病已被确定为一种特异性的 髓鞘相关糖蛋白或糖脂抗体;神经 通过电子显微镜对这些患者的活检进行研究 和免疫细胞化学。 淀粉样蛋白的性质 发现有“散发性”淀粉样多发性神经病的患者 利用淀粉样蛋白的特异性抗体 免疫细胞化学和生物化学对提取的 淀粉样蛋白 免疫细胞标志物在免疫过程中进行了研究。 在恒河猴中诱导的EAN和EAE的演变， 尝试使用一些新的免疫调节疗法， 剂. 猴炎性肌病的发病机制 免疫缺陷（猿猴艾滋病）由于逆转录病毒D，（SRV- （1）进一步研究。 与以下物质发生免疫反应的抗SRV-1抗体 炎性细胞侵入肌纤维; 能够感染组织培养中的成肌细胞，而不施加 肌肉中的细胞病变效应。 多发性肌炎和炎症 在艾滋病患者中也发现了神经病变， 正在研究肌肉或神经损伤的机制， 原位杂交和免疫细胞化学。 的影响 从5岁到25岁的猴子的神经肌肉系统的老化是 正在进行详细的形态学研究， 对他们的肌肉和神经活检进行形态计量分析。 自身免疫小鼠肌肉的形态学变化是 还研究了;管状聚集体被发现，它们与 正在研究内源性干扰素。

Enzyme histochemistry in muscle and nerve biopsies is carried out for diagnostic purposes in patients with several neuromuscular disorders. Immunocytochemical studies were conducted using specific antibodies to thymic peptides to investigate changes in the distribution of epithelial cells in the thymus of patients with myasthenia gravis. The interaction between cells of the lymphoid and central nervous system was investigated searching for common antigenic markers on their cell surface. Thymosin beta4, an immunomodulating polypeptide, was found to be a common antigen shared by macrophages, dendritic lymphoid cells and oligodendrocytes. The IgM of certain patients with paraproteinemic polyneuropathies has been identified as a specific antibody to myelin associated glycoprotein or glycolipids; nerve biopsies from these patients are studied by electron microscopy and immunocytochemically. The nature of amyloid protein in patients with "sporadic" amyloid polyneuropathy was identified using specific antibodies to amyloid proteins immunocytochemically and biochemically on the extracted amyloid. Immune cellular markers were investigated during the evolution of EAN and EAE induced in rhesus monkeys and therapies were attempted using some novel immunomodulating agents. The mechanism of inflammatory myopathy in monkeys with immunodeficiency (Simian AIDS) due to a retrovirus D, (SRV- 1), was further studied. Antibodies to SRV-1 immunoreacted with the inflammatory cells invading the muscle fibers; SRV-1 was capable of infecting myoblasts in tissue culture without exerting a cytopathic effect in the muscle. Polymyositis and inflammatory neuropathies were also identified in patients with AIDS and the mechanism of muscle or nerve damage is being investigated with in situ hybridization and immunocytochemistry. The effect of aging on the neuromuscular system of monkeys from age 5 to 25 is being investigated with a detailed morphological and morphometrical analysis of their muscle and nerve biopsies. Morphological changes in the muscles of autoimmune mice are also studied; tubular aggregates were found and their relation to endogenous interferon is being investigated.