MUTATIONS IN CONTROL REGIONS THAT REGULATE SV40 TRANSCRIPTION

SV40中的400个碱基对区域含有早期和晚期启动子元件， 晚期转录和DNA复制的起点。 我们已经确定了一 替代TATA盒，位于5'起始位点上游30个碱基对 在体外和体内发挥作用以调节 后期转录水平。 早期转录水平受到影响 通过富含GC的序列和它们与位于 在早期5'起始位点的上游。

A 400 base pair region in SV40 contains the promoter elements of early and late transcription and the origin of DNA replication. We have identified a surrogate TATA box that lies 30 base pairs upstream of the 5' start site of late transcription that functions in vitro and in vivo to regulate the level of late transcription. The level of early transcription is effected by GC rich sequences and their distance from the TATA box that lies upstream of the early 5' start site.