INTERACTION BETWEEN POLYMERIZED HUMAN ALBUMIN AND HEPATITIS B VIRUS

建立了一种竞争性抑制放射免疫分析法， 定量分析与HBsAg结合的聚合人血白蛋白。 基于该测定，我们已经表明，与戊二醛的特异性结合 聚合白蛋白比人白蛋白强至少1,000倍 通过其它程序如加热、老化和碳化二亚胺聚合 交联。 聚合白蛋白的特异性结合位点是 定位于HBsAg的pre-S基因产物。 一种测定方法， 已经开发了针对聚合白蛋白的抗体活性。

A competitive inhibition radioimmunoassay has been developed for quantitative analysis of the HBsAg-binding polymerized human albumin. Based on this assay, we have shown that specific binding for glutaraldehyde polymerized albumin is at least 1,000 fold stronger than human albumin polymerized by other procedures such as heat, aging and carbodiimide cross-linking. The specific binding site for polymerized albumin is localized on the pre-S gene product of HBsAg. An assay procedure for antibody activity to polymerized albumin has been developed.