MOLECULAR GENETIC ANALYSIS OF FELINE CELLULAR GENES--A COMPARATIVE APPROACH

家猫作为遗传分析动物模型的发展 开始于构建一个基因图谱，现在包括50多个 的位点 人类和猫科动物基因图谱的一个显著的连锁同源性是， 细胞学程序证实，今天超过25%的人类基因组 可以与猫科动物的染色体组型进行条带比对。 分型血清 猫主要组织相容性复合体通过同种异体 皮肤移植和血清学试剂的评价采用人群聚类法 分析和免疫沉淀的猫淋巴细胞抗原。 的 猫MHC复合物的分子结构是用 同源基因克隆并与人和鼠MHC簇进行比较。 哺乳动物原癌基因家族的进化已经被研究， 在猫、老鼠和人类中绘制这些基因， 历史 两个内源性逆转录病毒家族（RD-114和FeLV）已被发现。 从分子的角度进行了研究，似乎代表了 古老的感染和基因扩增。 一个进化 用两种方法构建了37种猫科动物的树 遗传距离的分子测量。

The development of the domestic cat as an animal model for genetic analysis began with the construction of a genetic map which now consists of over 50 loci. A striking linkage homology of the human and feline gene maps was confirmed by cytological procedures and today over 25% of the human genome can be aligned band for band with the feline karyotype. Typing sera for the feline major histocompatibility complex were generated by allogenic skin grafting and serological reagents were evaluated by population cluster analysis and by immunoprecipitation of feline lymphocyte antigens. The molecular organization of the feline MHC complex was derived using homologous gene clones and compared to the human and murine MHC clusters. Evolution of the proto-oncogene family of mammals has been studied by mapping these genes in cat, mouse and man, and reconstructing their natural history. Two endogenous retroviral families (RD-114 and FeLV) have been studied from a molecular perspective and appear to represent vestiges of ancient infections and gene amplification in the Felidae. An evolutionary tree of 37 species of the cat family has been constructed using two molecular measures of genetic distance.