NEUROREGULATORY ASPECTS OF NEUROMEDIN B

神经介肽B是最初从脊髓分离的神经肽， 属于Ranatensin/litorin肽家族， 铃蟾肽 制备了兔抗神经介肽B的异源抗血清 并表现出广泛的特异性：一种抗血清具有绝对的 对羧基末端四肽的特异性，因此交叉反应 对蛙皮素和litorin同样有效，但对蛙皮素无效;其他抗血清 对这些肽有轻微的识别（0.1-2%）。 所有的抗血清 与共享羧基末端Met-NH 2的物质P交叉反应 残余物 神经介肽B相关的适宜提取条件 肽显示，无论是酸性或碱性条件下产生的 最高浓度来自脊髓，而掺入 有机溶剂的萃取方法显着降低值 得到了

Neuromedin B is a neuropeptide originally isolated from spinal cord and belongs to the ranatensin/litorin family of peptides which are related to bombesin. Heterologous antisera against neuromedin B was raised in rabbits and exhibited a wide range of specificities: one antiserum had an absolute specificity for the carboxy terminal tetrapeptide, thus cross-reacting equally well with ranatensin and litorin, but not bombesin; other antisera had slight recognition (0.1-2%) of these peptides. None of the antisera cross-reacted with substance P which shares the carboxy terminal Met-NH2 residue. The appropriate extraction conditions for neuromedin B-related peptides revealed that either acidic or basic conditions yielded the highest concentrations from spinal cord, whereas the incorporation of organic solvents in the extraction method markedly reduced the values obtained.