INTRUMENTATION DEVELOPMENT FOR FLUORESCENCE SPECTROSCOPY

介绍了时间分辨荧光分光光度计的自动控制 开发用于快速收集荧光光谱。 几 基于激光的系统的外围设备由惠普公司的 1000系列微型计算机，包括采集、传输和存储 的数据，样本的选择，和自动定位， 单色器用于波长选择。 对这些外围设备的控制 允许比手动更快地收集数据 系统，很少或没有操作员干预，也允许最大 实验设计和改进的灵活性。 结合计算机控制和采集的特定实验 包括在采样和参考灯数据采集之间交替， 固定波长以获得荧光衰减曲线，并收集 在快速扫描时从样品获得时间分辨荧光数据 通过一系列的波长。 由于使用了模块化编程， 实验可以很容易地配置。

Automatic control of a time-resolved fluorescence spectrophotometer was developed to enable the rapid collection of fluorescence spectra. Several peripherals of the laser based system are controlled by a Hewlett-Packard Series 1000 mini-computer, including the acquisition, transfer, and storage of data, the selection of samples, and the automatic positioning of monochromators for wavelength selection. The control of these peripherals allows data to be collected more quickly than is possible with a manual system, with little or no operator intervention, and also allows maximum flexibility in the design and enhancement of experiments. Specific experiments that incorporate computer control and acquisition include alternating between sample and reference lamp data acquisition at fixed wavelengths to achieve fluorescence decay curves, and collecting time-resolved fluorescence data from a sample while rapidly scanning through a series of wavelengths. Because modular programming was used, new experiments can be easily configured.