MBRS PROGRAM AT WAYNE STATE UNIVERSITY

鲎变形细胞溶解物（LAL）检测试剂用于制备1 h 新鲜绞肉中内毒素的测定。 然后，这些值为 用于计算革兰氏阴性菌的总数， 鲎试剂测定的内毒素。 革兰氏阳性菌的估计数量 是由鲜肉中的预定比例决定的， 革兰氏阴性菌和革兰氏阳性菌。 因此， 用鲎试剂可在1 h内测定新鲜绞肉样品的含量。 研究 在整个项目的这一阶段， 绞肉中的内毒素及其聚丙烯酰胺凝胶法的分离。 肉源性微生物对肉内毒素影响的初步研究 肉类起源将启动。 第1部分的5.

The Limulus amoebocyte lysate (LAL) test reagent is used to make one h determinations of endotoxins in fresh ground meats. These values are then employed to calculate the total number of gram negative bacteria represented by the LAL-determined endotoxin. The estimated numbers of gram positive bacteria are determined by pre-determined ratios that exist in the fresh meats between gram negative bacteria and gram positives. Thus, the aerobic plate count of a fresh ground meat sample can be estimated in one h by use of LAL. Research during this phase of the overall project will focus on methods for extracting endotoxins from ground meats and their resolution by polyacrylamide gel methods. Initial studies on the effect of meat-borne microorganisms on endotoxins of meat origin will be initiated. Part 1 of 5.