CHEMISTRY/BIOLOGY OF THE GENETICALLY-DISTINCT COLLAGENS

这个项目的主要目标是阐明生物化学和 中阐述了遗传上不同的胶原蛋白的生物学特性 脊椎动物的结缔组织基质。这些研究 涉及：1)从各种组织中提取胶原蛋白；2)开发 选择性沉淀和层析技术允许 单个胶原蛋白分子种的分离纯化；3) 关于链组成的分子物种的表征 以及隔离链的化学性质；以及4)生产 针对不同分子物种的特异性多克隆抗体 用免疫组织化学和免疫组织化学方法评价它们的组织分布 免疫电子显微镜。 利用所指出的理论基础和方法，该方案已经 有助于辨别独特的生化特性和潜力 I、II、III、IV、V和VI型胶原的生理作用。不是主要的 当前研究工作的方向或重点的转变是 在这个时候考虑的。在这方面，该委员会的意图是 调查人员将解决与该事件相关的几个关键剩余问题 胶原蛋白家族的分子多样性，重点是 关于几种最近描述的胶原蛋白的研究 通常以相对少量的形式存在于各种组织中。

The major objectives of this program are to elucidate the biochemical and biological properties of the genetically-distinct collagens elaborated in the connective tissue matrices of vertebrate organisms. These studies involve: 1) extraction of collagens from various tissues; 2) development of selective precipitation and chromatographic techniques allowing the isolation and purification of individual molecular species of collagen; 3) characterization of the molecular species with respect to chain composition and chemical properties of the isolated chains; and 4) the production of specific polyclonal antibodies to various molecular species in order to assess their tissue distribution by immunohistochemistry as well as by immunoelectron microscopy. Utilizing the indicated rationale and approaches, this program has been instrumental in discerning the unique biochemical properties and potential physiological roles for type I, II, III, IV, V and VI collagens. No major shifts in direction or emphasis of the current research effort are contemplated at this time. In this regard, it is the intention of the investigators to address several key remaining issues relevant to the molecular diversity of the collagen family of proteins, with emphasis on studies concerning several of the more recently-described collagens which are generally present in relatively small quantities in various tissues.