MEMBRANE DAMAGE BY IMMUNE MECHANISMS

大鼠大颗粒淋巴细胞瘤细胞质颗粒的纯化 已经研究了NK活性，以确定它们在细胞毒性和 这些淋巴细胞的其他功能。 生物化学纯化 来自颗粒的溶细胞素显示它是一条单链的多肽链， 约65kD。 当红细胞被溶细胞素溶解时， 在已经纯化的膜中形成大的孔状复合物。 溶细胞素活性显示被多种脂质类似物阻断， 与膜插入机构一致。 抗独特型抗体 针对磷酸胆碱结合的骨髓瘤蛋白TEPC 15产生的抗体， 显示与纯化的溶细胞素结合并阻断其裂解活性， 表明胆碱被溶细胞素识别。 生物活性 还研究了颗粒的非溶细胞素组分。 这是 表明DNA酶活性与Percoll中的LGL颗粒相关， 梯度离心 该活性不是溶细胞素的一部分。 dna酶活性 具有中性pH值最佳值，并且在 非细胞毒性淋巴细胞。 与其他实验室的合作研究 其他颗粒成分的鉴定，包括一个因素， 对大颗粒淋巴细胞和其他细胞的趋化性，以及 嗜铬粒蛋白是一种以前只存在于神经内分泌细胞中的抗原， 颗粒。

The cytoplasmic granules purified from rat large granular lymphocyte tumors with NK activity have been studied to determine their role in cytotoxic and other functions of these lymphocytes. Biochemical purification of the cytolysin from the granules shows that it is a single peptide chain of about 65 kd. When erythrocytes are lysed with cytolysin, this molecule forms a large pore-like complex in the membrane which has been purified. Cytolysin activity was shown to be blocked by a variety of lipid analogues, in agreement with a membrane insertion mechanism. Anti-idiotype antibodies raised against the phosphorlycholine binding myeloma protein TEPC 15 were shown to bind to purified cytolysin and block its lytic activity, suggesting a choline recognition by cytolysin. The biological activities of non-cytolysin components of the granules were also studied. It was shown that a DNase activity is associated with LGL granules in the Percoll gradient. This activity was not part of the cytolysin. The DNAse activity has a neutral pH optimum and is found in much lesser amounts in non-cytotoxic lymphocytes. Collaborative studies with other labs have led to identification of other granule components, including a factor which is chemotactic to large granular lymphocytes and other cells, and chromogranin, an antigen previously known only in neuro-endocrine cell granules.