ORGANIZATION AND CONTROL OF GENETIC MATERIAL IN PLASMACYTOMAS

本课题的长远目标是研究控制问题 在调节细胞生长，肿瘤转化， 以及免疫系统的正常和恶性细胞中的蛋白质合成。 为此，我们正在研究正常组织中原癌基因的结构， 以及小鼠和人的肿瘤组织和这些癌基因的表达 作为mRNA。 我们特别关注小鼠浆细胞瘤， 骨髓性肿瘤和淋巴肉瘤，我们研究什么作用， Abelson和Moloney白血病病毒在诱导这种肿瘤中发挥作用 以及细胞内致癌基因的改变。 我们发现这些 病毒诱导一种形态上不同的肿瘤亚群（ABPL）， 由于插入了缺失形式的莫洛尼， 白血病病毒的myb基因。 这是一个哺乳动物的例子， 通过病毒的启动子/增强子插入激活癌基因。 关于ABPL 肿瘤现在似乎是骨髓源性的，所以我们正在研究myb 其他小鼠骨髓性肿瘤中的基因。 c-myc的表达是 在浆细胞瘤中增加，在某些浆细胞瘤中有染色体 易位只是5'的myc外显子I，RNA转录利用 两个myc启动子的比例与正常细胞不同。 两个这样 浆细胞瘤是myc RNA的超级生产者， 这种增强RNA合成的机制的线索。 我们也 研究癌基因在小鼠和人类自身免疫性疾病中的表达， 以及它们在小鼠和人类自身免疫中癌基因的表达， 疾病，以及它们在正常淋巴细胞中的表达， 有丝分裂原 某些类型的自身免疫性疾病的特征是高免疫性。 myc和raf RNA水平，而其他人的myb RNA水平升高。

It is the long range purpose of this project to study the control mechanisms important in regulating cell growth, neoplastic transformation, and protein synthesis in normal and malignant cells of the immune system. To this end we are studying the structure of the proto-oncogenes in normal and tumor tissues from mouse and man and the expression of these oncogenes as mRNAs. In particular we are focusing on mouse plasmacytomas, myelogenous tumors and lymphosarcomas, and we investigating what role Abelson and Moloney leukemia viruses play in the induction of such tumors and the alteration of cellular ongogenes. We have discovered that these viruses induce a morphologically distinct subset of tumors (ABPLs) which have altered myb mRNAs owing to the insertion of a deleted form of Moloney leukemia virus in the myb gene. This represents a mammalian example of oncogene activation by promoter/enhancer insertion of virus. The ABPL tumors now appear to be of myelogenous origin, so we are studying the myb genes in other mouse myelogenous tumors. The expression of c-myc is increased in plasmacytomas, and in certain plasmacytomas with chromosomal translocations just 5' of the myc exon I, RNA transcription utilizes the two myc promoters in a different ratio than in normal cells. Two of these plasmacytomas are super producers of myc RNA and are being studied for clues to the mechanism of this enhanced RNA synthesis. We are also studying the expression of oncogens in mouse and human autoimmune diseases, as well as their expression of oncogenes in mouse and human autoimmune diseases, as well as their expression in normal lymphocytes activated by mitogens. Certain classes of autoimmune diseases are characterized by high levels of myc and raf RNA, while others have elevated levels of myb RNA.