DEVELOPMENT OF REPRODUCTIVE-ENDOCRINE-GENETIC STRATEGIES IN ANIMAL SPECIES

该计划的目标是增加 非驯养野生动物生殖-内分泌-遗传数据库 物种 方案方法强调调查基本的 生殖-内分泌-遗传因素似乎是最关键的 人工育种策略应用的先决条件。 一 针对女性和男性生殖的多学科方法， 遗传学被利用了。 使用家养动物模型可以进行测试 研究概念在适应更稀有、非国内的 物种 女性的努力领域包括激素评估， 垂体-卵巢功能的内分泌特征的相关性， 操作压力影响指数;通过 外源性促性腺激素的管理，以优化的时机， 排卵事件;以及胚胎采集、培养、冷冻和移植 基因库技术，提高繁殖潜力 并最终允许显微注射分子克隆基因， 参与转化和先天缺陷。 特别强调 适用于采集、体外培养、冷冻， 小鼠、猫和小型猪、动物胚胎显微操作 稀有物种和人类疾病研究的模型。 努力领域 雄性包括精液收集和评价以表征射精 标准，并将这些发现与遗传多态性水平相关联， 野生动物种群;精液处理和冷冻保存以增加 精子活力和建立最佳方法长期储存 遗传物质;和激素评估，以提高对 垂体-性腺-肾上腺的关系，特别强调 在分类学上相关的野生动物之间的应激反应的显著差异 物种 总之，这些广义的研究概念允许快速 扩大稀有物种的生理遗传标准，并提高 评估生育潜力或遗传状态以优化 选择性自然复盖的管理努力。

The objective of this program is to increase the reproductive-endocrine-genetic data base of nondomesticated, wildlife species. Program approaches emphasize investigation of basic reproductive-endocrine-genetic factors which appear as the most critical prerequisites to the application of artificial breeding strategies. A multidisciplinary approach targeted toward female and male reproduction and genetics is employed. The use of domestic animal models permits testing the efficacy of research concepts before adaptation to rarer, nondomestic species. Areas of effort in the female include hormonal evaluation for correlation of endocrine profiles of pituitary-ovarian function and as an index of the influence of manipulative stress; ovulation induction through the administration of exogenous gonadotropins to optimize the timing of the ovulatory event; and embryo collection, culture, freezing and transferas techniques for cryobanking genetic stock, improving reproductive potential and eventually allowing microinjection of molecularly cloned genes which participate in transformation and inborn errors. Particular emphasis has been applied to the collection, in vitro culture, freezing, and micromanipulation of embryos of mouse, cat and miniature swine, animal models for rare species and the study of human disease. Areas of effort in the male include semen collection and evaluation to characterize ejaculate norms and correlate these findings to the level of genetic polymorphism in wildlife populations; semen handling and cryopreservation to increase spermatozoal viability and establish optimal methods for chronic storage of genetic material; and hormonal evaluation to improve the understanding of pituitary-gonadal-adrenal relationships with particular emphasis on the marked differences in stress responses among taxonomically related wildlife species. Together, these generalized research concepts permit rapid expansion of physiological-genetic norms for rare species, and also improve methods of assessing fertility potential or genetic status to optimize management efforts for selective natural progagation.