EVOLUTIONARY RELATIONSHIPS OF THE FELIDAE--A MITOCHONDRIAL DNA APPROACH

本研究的目的是对中国与西方的文化差异进行深入的比较研究， 猫科动物的染色体、蛋白质和DNA水平。 然而，猫科动物（Felidae）的生殖发育是不确定的。 线粒体DNA（mtDNA）已被选为进化探针。 的 分子进化迅速，是母系遗传，并提供了一个高水平的 近缘物种间的分辨度。 mtDNA已被 从家猫（10只）、狮子、猎豹（3只）中分离出 云豹（2只）和云豹（2只）。 除了有 在上述四个物种中，线粒体DNA已从美洲狮， 豹子和南美杰弗里的猫。线粒体DNA已经被 用17种不同的限制性内切酶消化， 为其中的五种动物（狮子、猎豹、家猫、云豹）建造的 杰弗里的猫（Geoffrey's Cat） 这五个物种的种内和种间变异 已对绘制的物种（“指标物种”）进行了测量。 的mtDNA。 家猫已被分子克隆到质粒pBR322中。 的mtDNA 克隆将被用作来自每个猫科物种的mtDNA的分子探针 (35的高分子量DNA的“Southern”分析， 培养的细胞系。 本文报道了中国对虾线粒体DNA的比较限制性酶切图谱， 不同的物种将被用来构建一个分子遗传学的 猫科动物 这种系统发育将比较系统发育拓扑学的 通过其他分子和形态测量得出的猫科动物。

The aim of this investigation is to make in-depth comparative studies at the chromosomal, protein and DNA levels among members of the cat family. However, the phylogeny of the cat family (Felidae) is uncertain. Mitochondrial DNA (mtDNA) has been chosen as an evolutionary probe. The molecule evolves rapidly, is maternally inherited and provides a high degree of resolution among closely related species. mtDNA has been isolated from the domestic cat (ten individuals), lion, cheetah (three individuals), and clouded leopard (two individuals). In addition to the four aforementioned species, mtDNA has been isolated from a cougar, a leopard and the South American Geoffrey's cat. The mtDNAs have been digested with 17 different restriction enzymes and cleavage maps have been constructed for five of them (lion, cheetah, domestic cat, clouded leopard and Geoffrey's cat). The intra- and interspecific variations of these five mapped species ("index species") have been measured. The mtDNA of the domestic cat has been molecularly cloned in the plasmid, pBR322. The mtDNA clones will be used as molecular probes of mtDNA from each Felidae species (35 of 37) using "Southern" analysis of high molecular weight DNA from cultured cell lines. The comparative restriction maps of the mtDNA from the various species will be used to construct a molecular phylogeny of the Felidae. This phylogeny will be compared to phylogenetic topologies of the Felidae derived by other molecular and morphometric measurements.