NEUROMUSCULAR AND CENTRAL NERVOUS SYSTEM DISEASES AND THEIR EXPERIMENTAL MODELS

在肌肉和神经活检中进行酶组织化学， 用于诊断患有多种神经肌肉疾病的患者。 使用特异性抗体进行免疫细胞化学研究， 胸腺肽研究上皮细胞分布的变化 重症肌无力患者胸腺中的细胞。 使用 细胞荧光照片，携带胸腺标记物的特定淋巴细胞亚群 （胸腺素α 1、α 7、β 4）。 之间的相互作用 研究了淋巴和中枢神经系统的细胞， 寻找细胞表面的共同抗原标记。 胸腺素β 4， 免疫调节多肽，被发现是一种共同的抗原， 巨噬细胞、树突状淋巴细胞和少突胶质细胞。 IgM抗体 某些患有副蛋白血症性多发性神经病的患者 作为髓鞘相关糖蛋白或糖脂的特异性抗体; 通过电子显微镜研究来自这些患者的神经活检， 免疫细胞化学 淀粉样蛋白的性质 “散发性”淀粉样多发性神经病是用特异性抗体鉴定的 淀粉样蛋白的免疫细胞化学反应， 对提取的淀粉样蛋白进行生化分析 免疫细胞标志物为 在恒河猴中诱发EAN和EAE的演变过程中研究 并尝试使用一些新的免疫调节剂进行治疗。 的 免疫缺陷猴炎性肌病的发病机制 艾滋病）由于逆转录病毒D，进一步研究。 抗体与 逆转录病毒与浸润肌肉的炎性细胞发生免疫反应 纤维;逆转录病毒能够感染组织培养中的成肌细胞 而不会在肌肉中产生细胞病变效应。 衰老对 正在研究5至25岁猴子的神经肌肉系统 对他们的肌肉进行了详细的形态学和形态测量学分析 和神经活检

Enzyme histochemistry in muscle and nerve biopsies is carried out for diagnostic purposes in patients with several neuromuscular disorders. Immunocytochemical studies were conducted using specific antibodies to thymic peptides to investigate changes in the distribution of epithelial cells in the thymus of patients with myasthenia gravis. Using the cytofluorograph, specific subsets of lymphocytes that carry thymic markers (thymosin alpha1, alpha7, beta4) were defined. The interaction between cells of the lymphoid and central nervous system was investigated searching for common antigenic markers on their cell surface. Thymosin beta4, an immunomodulating polypeptide, was found to be a common antigen shared by macrophages, dendritic lymphoid cells and oligodendrocytes. The IgM of certain patients with paraproteinemic polyneuropathies has been identified as a specific antibody to myelin associated glycoprotein or glycolipids; nerve biopsies from these patients are studied by electron microscopy and immunocytochemically. The nature of amyloid protein in patients with "sporadic" amyloid polyneuropathy was identified using specific antibodies to amyloid proteins immunocytochemically on tissue sections and biochemically on the extracted amyloid. Immune cellular markers were investigated during the evolution of EAN and EAE induced in rhesus monkeys and therapies were attempted using some novel immunomodulating agents. The mechanism of inflammatory myopathy in monkeys with immunodeficiency (Simian AIDS) due to a retrovirus D, was further studied. Antibodies to the retrovirus immunoreacted with the inflammatory cells invading the muscle fibers; the retrovirus was capable of infecting myoblasts in tissue culture without exerting a cytopathic effect in the muscle. The effect of aging on the neuromuscular system of monkeys from age 5 to 25 is being investigated with a detailed morphological and morphometrical analysis of their muscle and nerve biopsies.