SYNTHETIC ANALOGS OF ROTAVIRUS GENOMIC RNA EXPRESSING A FOREIGN MARKER GENE

轮状病毒基因组由11个双链片段组成 RNA(DsRNA)，编码6种结构蛋白(VP1、VP2、VP3、VP4、 VP6、VP7)和5种非结构蛋白(NS53、NS34、NS35、NS20、 和NS26)。每个基因组RNA片段都包含一个甲基化的5‘-帽 在5‘端测序M7GpppG(M)GPY。在以下位置未发现多聚腺苷酸链 3‘端和RNA转录本不是多腺化的。的5‘和3’端 每个基因组片段都包含不同的、不相关的末端共识 七到十个核苷酸的序列。他们被认为是重要的 顺式作用信号。末端也被认为含有序列 在包装和调节轮状病毒基因表达中的重要作用 在转录、复制和翻译水平上。然而， 末端在轮状病毒基因调控中的精确作用 表达是未知的，对顺式作用知之甚少 来自我们先前工作的信号表明3‘-末端顺式作用 复制所需的信号是19‘-末端，它 包括7-nT-末端共有序列和12nT的 相邻的、保守程度较低的序列[Gorziglia和Collins，1992]。

The genome of rotavirus consists of eleven segments of double-stranded RNA (dsRNA) which encode six structured proteins (VP1, VP2, VP3, VP4, VP6, and VP7) and five nonstructural proteins (NS53, NS34, NS35, NS20, and NS26). Each genomic RNA segment contains a methylated cap 5' - sequence M7GpppG(m)GPy at 5' end. No polyadenylation tract is found at 3' end and RNA transcripts are not polyadenylated. The 5' and 3' end of each genome segment contain distinct, unrelated terminal consensus sequence of seven to ten nucleotides. They are assumed to be important cis-acting signal. The termini are also thought to contain sequences important in packaging and in the regulation of rotavirus gene expression at the level of transcription replication, and translation. However, the precise roles of the termini in the regulation of rotavirus gene expression are not known, little has been learned about the cis-acting signal from our previous work that indicated the 3'-terminal cis-acting signals required for replication are the nineteen 3'-terminal which include the 7-nt-terminal consensus sequence together with 12nt of adjoining, less-well-conserved sequence [Gorziglia and Collins, 1992].