NEW TESTING FOR ALLERGENIC EXTRACTS

答：相对效价的酶联免疫吸附试验：一种酶联免疫吸附试验竞争试验已经 为过敏性提取物的标准化而开发。多数 过敏性提取物的制造商已经采用了ELISA竞赛 代替Rast抑制进行效力测试。与 这项检测的实施，我们降低了费用和 本实验室在过敏原检测中所花费的人员时间 产品发布前的销售和作为新的参考。 目前，正在进行测试白桦树花粉、乳胶和 美国蟑螂、德国蟑螂和东方蟑螂的ELISA竞赛 化验。 B.总蛋白测定法的改进：一种改进的吲哚 建立并验证了检测过敏原中蛋白质的方法 萃取物。这种方法减少了进行金刚三酮操作的人员时间 蛋白质减少了一半以上。过敏性产品的验证研究 检测实验室显示，吲哚三酮检测与 改良的金刚三酮测定法。该方法已被送往制造厂，并 其他检测实验室将其投入到两者的相关性中 方法：研究方法。来自实验室的数据将被分析。 C.用于效力测试的化学发光：Origen免疫分析系统 被评估为同等或更好的执行效力的方法 过敏提取物的测试。已确定受限制的 一次可以测试的试管数量会导致速度较慢 批次放行测试的周转时间。 D.过敏提取物的SDS-PAGE和Western blotting：研究如下 正在进行的是确定SDS-PAGE和蛋白质印迹技术 可与等电聚焦一起使用或替代等电聚焦和 变应原提取物和血清的BIEF-Light表征方法 来自过敏者。

A. ELISA for Relative Potency: An ELISA Competition Assay has been developed for the standardization of allergenic extracts. Most manufacturers of allergenic extracts have adopted the ELISA Competition Assay in place of the RAST Inhibition for potency testing. With the implementation of this assay, we have reduced the expense and the personnel time incurred by this laboratory in the testing of allergenic products prior to their release for sale and as new references. Currently, studies are proceeding to test birch tree pollen, latex, and American, German, and Oriental cockroaches using the ELISA Competition Assay. B. Modified Ninhydrin Assay for Total Protein: A modified ninhydrin assay was developed and validated for testing protein in allergenic extracts. This method reduces personnel time for performing ninhydrin proteins by more than half. Validation studies by the Allergenic Product Testing Lab have shown good correlation between the Ninhydrin Assay and the Modified Ninhydrin Assay. The method was sent to manufacturing and other testing laboratories for their input into correlation of the two methods. Data from the laboratories will be analyzed. C. Chemiluminescence for Potency Testing: The ORIGEN Immunoassay System was evaluated as an equivalent or better method for performing potency testing of allergenic extracts. It was determined that the restricted number of tubes that could be tested at one time would cause a slower turn-around time for lot release testing. D. SDS-Page and Western Blotting of Allergenic Extracts: Studies are being conducted to determine if SDS-Page and Western blotting techniques can be used in conjunction with or to replace IsoElectric Focusing and BIEF-Light methods for characterization of allergenic extracts and serum from allergic individuals.