MOLECULAR GENETIC ANALYSIS OF FELINE CELLULAR GENES--A COMPARATIVE APPROACH

已获得了100多个遗传位点的共线图， 19只猫中有17只含有I型（编码基因）标记的家猫 染色体 该地图是使用体细胞杂交面板构建的， 分辨率G显带核型，荧光原位杂交，和 系谱分析 猫与人连锁位置的比对 基因座揭示了猫科动物和人类的广泛同线性保守 基因组，在某些情况下跨越整个染色体。 深入 连锁分析和基因组组织（包括分子 克隆，基因定位，序列分析）， 以下基因家族：（1）弗拉，猫主要组织相容性 复合体;（2）猫科动物同源异型盒基因;（3）性别决定基因，SRY，ZFY 和ZFX;（4）内源性逆转录病毒家族，RD 114和FeLV;（5） 原癌基因和病毒整合位点;和（6）毛色基因。 在与领先的比较基因作图在小鼠和 牛，我们已经确定了321个I型（编码基因）标记锚位点 用于所有物种的比较绘图。 锚位点为 选择以提供5-10厘摩密度的基因标记以供使用 来比较所有哺乳动物的基因图谱。 在 为了构建猫的遗传连锁图谱，我们启动了 家猫和家猫之间的种间有性杂交和回交， 孟加拉豹（Prionailurus bengalensis）该物种被选中 因为进化上的遗传距离以及我们的能力 来制造能生育的F1代雌性。 生产了80多个F1个体， 主要通过人工授精，超过48个回交个体 诞生了 利用种间回交构建了一个 同源的猫B4染色体的比较锚位点图 人类12号染色体 猫DNA的M13文库已被用于 筛选高变短串联重复序列多态性（STRP）还 称为微卫星位点。 到目前为止，已经有300个被测序，聚合酶 设计了链式反应引物，目前正在绘制STRP标记 在物种间回交。

A syntenic map of over 100 genetic loci has been achieved for the domestic cat containing Type I (coding gene) markers on 17 of the 19 cat chromosomes. The map was built using somatic cell hybrid panels, high resolution G-banding karyology, fluorescence in situ hybridization, and pedigree analysis. Alignment of linkage position of feline with human loci revealed extensive syntenic conservation of the feline and human genomes, in several cases spanning entire chromosomes. In- depth analysis of linkage and genomic organization (including molecular cloning, gene mapping, sequence analysis) was undertaken for the following gene families: (1) FLA, feline major histocompatibility complex; (2) feline homeobox genes; (3) sex determination genes, SRY, ZFY and ZFX; (4) endogenous retroviral family, RD114 and FeLV; (5) proto-oncogenes and viral integration sites; and (6) coat color genes. In collaboration with leading comparative gene mappers in mouse and cattle, we have identified 321 Type I (coding gene) marker anchor loci for use in comparative mapping for all species. Anchor loci were selected to provide a 5-10 centiMorgans density of gene markers for use in comparing genomic segments for all mammalian species' gene maps. In order to construct a genetic linkage map of the cat, we initiated inter-species sexual crosses and back-crosses between domestic cat and Asian leopard cat (Prionailurus bengalensis). This species was selected because of a substantive evolutionary genetic distance plus our ability to make fertile F1 females. Over 80 F1 individuals were produced, primarily by artificial insemination, and over 48 back-cross individuals have been born. The interspecies back-cross was used to construct a comparative anchor locus map of feline chromosome B4 which is homologous to human chromosome 12. M13 libraries of cat DNA have been used to screen for hypervariable short tandem repeat polymorphism (STRP) also called microsatellite loci. To date 300 have been sequenced, polymerase chain reaction primers designed, and STRP markers are now being mapped on the interspecies back-cross.