CORE--ORGAN CULTURE AND TISSUE PROCESSING FACILITY

器官培养和组织处理核心设施的目的是 为生化、组织提供集中的后肾素来源 培养、原位杂交和免疫组织化学分析 四个参与实验室。后肾功能的获取与维护 需要相当多的技能和人员已经处理过的经验 设施的一部分。此核心将释放时间和资源 否则项目主管将不得不致力于组织准备工作 并推动他们沿着拟议的调查方向取得进展。 该设施将确保一致性，允许交叉比较 观察到的运输蛋白、生长相关基因和 因素和结构。核心设施将最大限度地减少 减少设备，减少开支。维护的集中化 后肾器官培养和组织处理将确保一致性和 在不同实验室中进行的观测的重复性。

The purpose of the organ culture and tissue processing core facility is to provide a centralized source of metanephroi for biochemical, tissue culture, in situ hybridization, and immunohistochemical analysis by the four participating laboratories. Obtaining and maintaining metanephroi entail considerable skill and experience already processed by the personnel of the facility. This core will free the time and resources that the project directors would have had to otherwise devote to tissue preparation and facilitate their progression along the proposed lines of investigation. The facility will assure uniformity, allowing cross comparisons of the timing of observed changes in transport proteins, growth related genes and factors, and structure. The core facility will minimize duplication of equipment and decrease expenditures. The centralization of maintaining metanephric organ culture and tissue processing will assure uniformity and reproducibility of observations made in separate laboratories.