MANNOLIPID BIOSYNTHESIS, TURNOVER AND TRANSPORT

拟议的实验评估了生物合成、周转和可能的 含甘露糖脂在动物细胞和酵母中的运输。一定的 包括磷脂酰肌醇(PI)在内的甘露糖脂成为膜 糖基磷脂酰肌醇(GPI)锚定蛋白的锚定。那些 其中包括多醇以天冬酰胺连接的形式贡献其葡聚糖 糖蛋白的糖链。 实验将分析： 1A)这两类货物的周转量和可能的运输 甘露脂， 1b)不完全甘露糖脂的周转和可能的运输 由突变细胞产生的结构， 2)突变细胞内化极性甘露糖脂的能力 细胞表面到RER，从而纠正GPI锚定缺陷， 3)不能合成乙醚类脂的突变细胞是否能够 PI-连接甘露糖脂或GPI-锚定的合成及可能的研究 任何此类缺陷在齐薇格综合征中的意义， 4)PI连接的甘露糖脂能否被转移到蛋白质受体上 当胰腺微粒体用一种蛋白质的mRNA编程时，这种蛋白质 通常有一个GPI主播。

The proposed experiments evaluate the biosynthesis, turnover and possible transport of mannose-containing lipids in animal cells and yeast. Certain mannolipids which include phosphatidylinositol (PI) become the membrane anchors of glycosyl phosphatidylinositol (GPI)-anchored proteins. Those which include dolichol contribute their glycans as asparagine-linked glycans of glycoproteins. Experiments will analyze: 1a) the turnover and possible transport of these two classes of mannolipids, 1b) the turnover and possible transport of mannolipids of incomplete structure produced by mutant cells, 2) the ability of mutant cells to internalize polar mannolipids from the cell surface to the RER, thereby correcting GPI-anchoring defects, 3) whether mutant cells which cannot synthesize ether lipids are able to synthesize PI-linked mannolipids or GPI-anchors and the possible significance of any such deficiency in Zellweger's syndrome, 4) whether PI-linked mannolipids can be transferred to protein acceptors when pancreatic microsomes are programmed with mRNA of a protein which normally bears a GPI anchor.