TRANSCRIPTION OF RAT RIBOSOMAL DNA

描述：这个项目涉及的机制包括 核糖体RNA合成的调控。哺乳动物的rdna启动子由 包括两个元件：核心启动子(CPE)和上游元件(UPE)。 它们至少与两个转录因子相互作用：UBF和SL1。SL1 与UPE和CPE结合，是体外转录所必需的。UBF 结合CPE，是获得最大转录活性所必需的 体外培养。工作假说认为，UBF会弯曲DNA，从而 近似SF1结合位点。RB阻止UBF激活并阻止 UBF与SL1的相互作用。UBF的表达受以下因素的调控 促进生长和分化，调节UBF的活性 通过磷酸化。本项目主要关注UBF的监管 这些过程的表达和活性及其在调控中的作用 RDNA转录。

DESCRIPTION: This project deals with the mechanisms that account for regulation of ribosomal RNA synthesis. The mammalian rDNA promoter consists of two elements: a core promoter (CPE) and an upstream element (UPE). These interact with at least two transcription factors: UBF and SL1. SL1 binds both UPE and CPE and is required for transcription in vitro. UBF binds the CPE and is necessary for maximum transcriptional activity in vitro. The working hypothesis holds that UBF bends the DNA so as to approximate the SF1 binding sites. Rb blocks activation by UBF and prevents interaction between UBF and SL1. The expression of UBF is regulated by growth stimulation and differentiation, and the activity of UBF is regulated by phosphorylation. This project focuses primarily on the regulation of UBF expression and activity and the role of these processes in regulation of rDNA transcription.