TRANSCRIPTION OF RAT RIBOSOMAL DNA

描述：本项目涉及的机制，占 核糖体RNA合成的调节。 哺乳动物rDNA启动子由 核心启动子（CPE）和上游元件（UPE）。 它们与至少两种转录因子相互作用：UBF和SL1。 SL1 结合UPE和CPE，并且是体外转录所需的。 UBF 结合CPE，并且是最大转录活性所必需的， 体外 工作假设认为，UBF弯曲DNA，以便 接近SF1结合位点。 Rb阻断UBF的激活， UBF和SL 1之间的相互作用。 UBF的表达受 生长刺激和分化，并调节UBF的活性 通过磷酸化。 该项目主要关注UBF的监管 表达和活性以及这些过程在调节 rDNA转录。

DESCRIPTION: This project deals with the mechanisms that account for regulation of ribosomal RNA synthesis. The mammalian rDNA promoter consists of two elements: a core promoter (CPE) and an upstream element (UPE). These interact with at least two transcription factors: UBF and SL1. SL1 binds both UPE and CPE and is required for transcription in vitro. UBF binds the CPE and is necessary for maximum transcriptional activity in vitro. The working hypothesis holds that UBF bends the DNA so as to approximate the SF1 binding sites. Rb blocks activation by UBF and prevents interaction between UBF and SL1. The expression of UBF is regulated by growth stimulation and differentiation, and the activity of UBF is regulated by phosphorylation. This project focuses primarily on the regulation of UBF expression and activity and the role of these processes in regulation of rDNA transcription.