WAVELET-BASED AUTOMATED CHROMOSOME IDENTIFICATION

商业自动核型分析仪器已经改进到这样的程度，其中限制通量的主要因素是操作员校正染色体分类错误所需的时间。染色体分类准确性的提高将显著增加这些仪器在细胞遗传学实验室中的价值。该项目的目标是开发和商业化显着改进的染色体测量和分类技术，用于自动核型分析。目前，性能最好的染色体分类方法使用加权密度分布（WDD）特征[11]来量化染色体的带型。这些被计算为条带轮廓和一组WDD基函数之间的内积。最初由Granum [11，38]提出的一组特殊函数已得到广泛使用。在第一阶段，我们发现存在更好的函数集，我们的新方法可以找到比目前使用的最好的WDD功能更好的WDD功能。我们有一个创新的基于小波的方法产生WDD功能和染色体分类测试床，支持大规模的分类实验。我们建议在第二阶段进行彻底的，有条理的搜索，以获得更好的基函数。第三阶段将把这项技术纳入PSI的PowerGene自动核型分析仪器。拟议的商业应用：当新的染色体分类技术有资格用于常规应用时，它将被纳入PSI的Powergene产品中，无论是在新的系统中销售还是作为现有系统的升级。

Commercial automated karyotyping instruments have improved to the point where the major factor limiting throughput is the time required for operator correction of chromosome classification errors. An improvement in chromosome classification accuracy would significantly increase the value of these instruments in cytogenetics labs. The goal of this project is to develop and commercialize significantly improved chromosome measurement and classification techniques for automated karyotyping. Currently the best-performing chromosome classification approach uses Weighted Density Distribution (WDD) features [11] to quantify the banding pattern of the chromosomes. These are computed as inner products between the banding profile and a set of WDD basis functions. The particular set of 1unctions originally proposed by Granum [11,38] has come into widespread use. In Phase I we showed that better function sets exist and that our new approach can find better WDD features than the best currently used. We have an innovative wavelet-based method for generating WDD functions and a chromosome classification testbed which supports large scale classification experiments. We propose to conduct a thorough, methodical search for better performing basis functions in Phase II. Phase III will incorporate the technology into PSI's PowerGene automated karyotyping instruments. PROPOSED COMMERCIAL APPLICATIONS: When the new chromosome classification technology is qualified for routine application, it will be incorporated into PSI's Powergene products, both in new systems sold and as an upgrade to existing systems.