CORE--MOLECULAR BIOLOGY

分子生物学核心将用于子项目1、2、4和6。 CORE将提供服务，极大地促进研究 由次级项目1和6提出，更重要的是，核心将允许 子项目2和4可以使用他们自己没有的方法学 实验室。访问这样的核心是至关重要的，因为实施 在每个研究人员的实验室里，这些技术都是令人望而却步的 很贵的。例如，Coronado博士(分项目2)希望获得 可靠和充足的某些毒素供应，来自墨西哥面包粉 可怕的赫罗德玛蜥蜴，将被用作解剖工具 兰尼定受体在心肌中的功能。服务 由分子生物学核心提供的将包括分离毒素 DNA，DNA序列测定和表达载体的构建 这将允许在细菌中产生毒素。表达方式 而这些重组毒素的纯化将由该蛋白质完成 生物化学核心(核心B)。科罗拉多博士既没有 在他自己的实验室里完成这项工作所需的设备或专业知识。 然而，这两个核心服务的使用将使该项目能够 及时完成。

The Molecular Biology Core will be used by subprojects 1, 2, 4, and 6. The core will provide services that will greatly facilitate the research proposed by subprojects 1 and 6, and more importantly, the core will allow subprojects 2 and 4 access to methodologies absent from there own laboratories. Access to such a core is essential because implementation of these technologies in each investigators' laboratory would be prohibitively expensive. For example, Dr. Coronado (subproject 2) wishes to obtain a reliable and abundant supply of certain toxins, from the Mexican breaded lizard Heloderma horridum horridum, that will be used as tools to dissect the function of the ryanodine receptor in cardiac muscle. Services provided by the Molecular Biology Core will include isolating the toxin cDNAs, determining the DNA sequence and constructing expression vectors that will permit the production of the toxins in bacteria. The expression and purification of these recombinant toxins will be done by the Protein Biochemistry Core (Core B). Dr. Coronado does not have either the equipment or the expertise to allow this to be done in his own laboratory. However, the use of the two core services will enable this project to be completed in a timely manner.