QUANTITATION OF CARDIAC MYOCYTE CONTRACTILE DYNAMICS

描述：(改编自申请人的摘要)：一些基本的 旨在了解老年人的生理学和病理生理学的问题 心肌现在正在细胞水平上得到解决。的主要目的是 这一第二阶段的应用是将收缩测量工具商业化 在研究心肌细胞的第一阶段发展起来的。这个 心肌细胞长度和肌节的数字成像定量方法 到目前为止，Spacing在实验室测试中表现得非常好。这个 研究人员提供了一些收集自心脏的数据的例子 心肌细胞以及经历快速维度的非心脏组织 改变。这些实验结果证明了该光学系统的可行性。 尺寸标注方法及其在设计中实施的威力。这个 调查人员已经获得了六个成熟的心肌研究实验室 作为我们系统的后原型版本的评估点。四 更多的实验室将把Ionoptix Corp.的S尺寸测量工具应用于各种 组织阵列，包括微血管、肾小管和成骨细胞。 这里所代表的研究兴趣的范围表明了一个广泛的商业 电池尺寸测量系统的市场。最后，对这些功能的增强 第二阶段拟议的系统，包括全面收缩 瞬变分析和同时荧光离子定量将进一步 这种乐器的商业吸引力。 建议的商业应用：不可用

DESCRIPTION : (Adapted from applicant's abstract): Some of the fundamental questions aimed at understanding the physiology and pathophysiology of the myocardium are now being addressed at the cellular level. The principal aim of this Phase II application is to commercialize contractility measurement tools developed during the Phase I period for the study of cardiac myocytes. The digital imaging approach for quantitation of myocyte cell length and sarcomere spacing has performed extremely well in laboratory tests done thus far. The investigators present a number of examples of data collected from cardiac myocytes as well as from non-cardiac tissues which undergo rapid dimensional changes. These experimental results demonstrate the feasibility of the optical dimensioning approach and the power of its implementation in the design. The investigators have secured six established cardiac muscle research labs to serve as evaluation sites for post-prototype versions of our systems. Four additional labs will apply Ionoptix Corp.'s dimensioning tools to a diverse array of tissues including blood micro vessels, renal tubules and osteolasts. The range of research interest represented here indicates a broad commercial market for the cell dimensioning systems. Finally, the enhancements to these systems that is proposed for Phase II, including comprehensive contractile transient analysis and simultaneous fluorescence ion quantitation will further the commercial appeal of this instrumentation. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE