ELECTROCHEMICAL BIOSENSOR OF CARDIAC MARKERS

建立了一种新型的免疫层析电化学生物传感器（IEB），用于同时测定心肌梗死诊断的重要指标肌钙蛋白I（Troponin-I）、肌红蛋白（Myoglobin）和肌酸激酶同工酶（CK-MB）。这种使用电化学检测器的生物传感器将避免当前免疫测定中的光学干扰，并且将提供更高的准确度和灵敏度以快速定量分析物。我们的初步结果表明，使用所提出的方法定量肌红蛋白的几个优点，目前存在的方法，如高灵敏度，重现性，快速和使用方便。我们建议进一步优化这种新的方法，同时定量上述心脏生物标志物，并开发一种便携式生物传感器在床旁护理应用。拟议的生物传感器将包括一个免疫层析条，它产生的免疫复合物，这些心脏标志物与他们的特定抗体标记不同的金属离子和电化学检测器，定量这些金属离子的复杂的。将仔细开发IEB的设计，还将评估和优化通过测试条中的电极同时定量金属离子。这种生物传感器将为正确的临床决策提供必要的信息，从而大大提高冠状动脉护理的质量。拟议的商业应用：美国每年进行约1600万次心脏检查。因此，迫切需要建立一种快速、特异性高、灵敏度高、临床准确的心肌标志物同时定量检测方法。所提出的免疫层析电化学生物传感器有望在急诊室或冠心病监护室提供心肌梗死诊断的这些特征。这样的生物传感器将提供低成本、高效率、临床精确和简单的操作而无需技术培训，因此将在商业应用中具有额外的潜力。

Development of a novel immunochromatographic electrochemical biosensor(IEB) is proposed for simultaneous quantitation of Troponin-I, Myoglobin and CK-MB, which have been reported to be important indexes in the diagnosis of myocardial infraction. Such biosensor using electrochemical detectors will avoid optical interference in the current immunoassays, and will provide more accuracy and sensitivity to rapidly quantify analytes. Our preliminary results using the proposed methodology for quantitation of myoglobin demonstrated several advantages over the current existed method such as high sensitivity, reproducibility, rapidity and convenience in use. We propose to further optimize this novel method for simultaneous quantitation of the above mentioned cardiac bio-markers, and develop a portable biosensor in point-of-care application. The proposed biosensor will consist of an immunochromatographic strip which produces immuno-complexes of these cardiac markers with their specific antibodies labeled with different metal ions and an electrochemical detector which quantifies the amounts of these metal ions in the complex. Design of the IEB will be carefully developed, and simultaneous quantitation of metal ions by the electrode in the test strips will also be evaluated and optimized. Such biosensor will provide essential information for proper clinical decision, and thus greatly improve the quality of coronary care. PROPOSED COMMERCIAL APPLICATIONS: About 16 million cardiac examinations were conducted annually in the United States. A method with rapidity, high specificity and sensitivity, clinical accuracy is urgent to be developed for simultaneous quantitation of the cardiac markers in coronary care. The proposed immunochromatographic electrochemical biosensor is expected to provide these characteristics of myocardial infarction diagnosis at emergency room or coronary care units. Such biosensor will provide a low cost, highly efficient, clinically accurate and simple operation without technical training, and thus will have additional potential in commercial applications.