ELECTROCHEMICAL MEASUREMENT OF S-NITROSOTHIOLS

拟议项目的长期目标是创造一种方法和仪器，用于快速测量s -亚硝基硫醇化合物的浓度，并具有能够在生物学相关范围内检测这些物种的灵敏度。s -亚硝基硫醇具有广泛的生物学功能，其中一些是作为一氧化氮（NO）供体的化合物，另一些是通过其他分子机制来解释的。这些化合物很重要，因为它们构成了一氧化氮的重要内源性生物储存。s -亚硝基硫醇的测量将通过精确测量这些化合物受控分解释放的NO来实现。一氧化氮与自由基的重要和多样的相互作用现在在健康科学中是众所周知的。拟议的项目将创新最先进的一氧化氮传感器，以便在血液和血浆中检测浓度为1纳摩尔或更低的S-亚硝基硫醇。如果成功，预计预计的仪器将很容易被世界各地的研究人员接受。拟议的商业应用：预计随着生物科学研究界对NO的生理和病理生理作用的巨大和持续的兴趣；亚硫硝酸盐的测定在医学诊断和治疗中的作用将很快显现出来。这将最终确保这项事业的商业成功。

The long term goal of the proposed project is to create method and instrumentation with which to measure, the concentration of S-nitrosothiol compounds rapidly and with a sensitivity capable of detecting these species in the biologically relevant range. S-nitrosothiols have a broad range of biological functions some best explained by the compounds acting as nitric oxide (NO) donors and others through other molecular mechanisms. These compounds are important because they constitute a significant endogenous biological store of NO. Measurement of S-nitrosothiols will be effected by accurate measurement of NO released by controlled decomposition of these compounds. The important and diverse interactions of NO with free radicals are now well known in the health sciences. The proposed project will innovate new state of the art NO sensors so as to offer detection of S- nitrosothiols at concentrations of 1 nanomolar or less in blood and plasma. If successful, it is anticipated the projected instrumentation will be readily accepted by researchers worldwide. PROPOSED COMMERCIAL APPLICATIONS: It is expected that with the great and continued interest from the bioscience research community in the physiological and pathophysiological role of NO; a role for the measurement of thionitrites in medical diagnosis and treatment will emerge very soon. This would ultimately assure the commercial success of this undertaking.