WAVELET ENHANCEMENT OF CHROMOSOME BANDING PATTERNS

该项目旨在开发和商业化大大改进的软件，以数字方式增强显微图像中染色体带型的细节。这些研究人员已经开发出一种创新的技术，这一应用，小波变换和多分辨率图像分析的基础上。与现代计算机化的染色体分析所提出的技术承诺显着提高增强染色体带型和更有效的视觉检测的细微重排。这将有助于临床医生和研究人员检测以前不可见或亚可见的带模式的改变，在传统和高分辨率带。它将显著提高自动化仪器的能力，以帮助评估临床样本和正常及肿瘤哺乳动物细胞中的染色体改变。在第一阶段，我们实施和测试三个小波变换所需的数学特性。我们开发了一个原型多分辨率图像处理系统的染色体增强。我们得到了非常令人鼓舞的结果，强烈表明，这些技术提供了显着改善的增强能力比传统的方法。并清楚地证明了这种方法的可行性。在第二阶段，我们将完成软件的实施和改进。我们将实现几种小波设计方法，并评估许多小波变换基函数集，这些基函数集可能会带来染色体带型的相关细节。拟议的商业应用：一旦新的增强技术被开发出来并有资格用于常规应用，它们将被纳入PSII的PowerGene产品，无论是在新的系统销售还是作为现有系统的升级。

This project aims to develop and commercialize significantly improved software for digital enhancement of the detail of chromosome banding patterns in microscopic images. These investigators have developed an innovative technique for this application, based upon wavelet transforms and multiresolution image analysis. Used with modern computerized chromosome analysis the proposed technique promises significantly improved enhancement of chromosome banding patterns and more effective visual detection of subtle rearrangements. This will help clinicians and researchers detect previously invisible or sub-visible band pattern alterations in conventional and high resolution banding. It will significantly increase the ability of automated instruments to assist the evaluation of chromosome alterations in clinical samples and in normal and neoplastic mammalian cells. During Phase I we implemented and tested three wavelet transforms with desirable mathematical properties. We developed a prototype multiresolution image processing system for chromosome enhancement. We obtained extremely encouraging results, strongly suggesting that these techniques offer considerably improved enhancement capability over conventional methods. and clearly demonstrating the feasibility of this approach. In Phase II we will complete the implementation and refinement of the software. We will implement several wavelet design approaches and evaluate many wavelet transform basis function sets that potentially can bring out relevant detail in chromosome banding patterns. PROPOSED COMMERCIAL APPLICATIONS: As soon as the new enhancement techniques are developed and qualified for routine application, they will be incorporated into PSII's PowerGene products, both in new systems sold and as an upgrade to existing systems.