CORE--TRANSGENIC MOUSE FACILITY

这项计划拨款反映了概念和技术上的交织 一系列实验，以了解细胞信号的生物学和 阐明信号中的扰动所引起的分子事件 途径可能导致正常细胞向恶性细胞的转化 表型。恶性表型稳定的观察 遗传，导致了大量基因的识别 参与细胞信号转导，其表达改变导致细胞 转型。分子生物学和遗传学的新技术， 产生特定原癌基因突变的小鼠的能力，如 以及异常表达这些基因的转基因小鼠的产生 基因，现在可以分析这些遗传元素的功能 在组织层面上。这提供了新的和重要的见解 这些基因在正常发育中的作用，以及这些基因在 它们的功能改变可能会导致恶变。AS 因此，这个计划中的建议严重依赖于基因 同源基因打靶的小鼠操控 重组或转基因小鼠的产生。

This Program grant reflects a conceptually and technically intertwined series of experiments to understand the biology of cell signaling and to elucidate the molecular events by which perturbations in signaling pathways may lead to the conversion of a normal cell to the malignant phenotype. The observation that the malignant phenotype is stably inherited, has lead to the identification of a vast array of genes involved in cell signaling whose altered expression leads to cellular transformation. New technologies in molecular biology and genetics, the ability to generate mice with mutations in specific proto-oncogenes, as well as the generation of transgenic mice aberrantly expressing these genes, now permits an analysis of the function of these genetic elements at an organismic level. This has provided new and significant insight into the role of these genes in normal development, and the way in which alteration in their function may lead to malignant transformation. As such, the proposals in this program rely heavily on the genetic manipulation of mice involving either gene targeting by homologous recombination or the generation of transgenic mice.