CORE--MOLECULAR BIOLOGY

慈惠文化核心设施对此至关重要 计划项目，因为他们将继续提供培养细胞， 受EB影响的个人，来自其未受影响的家庭成员，以及 来自无关健康对照组，用于所有项目的研究。 期间 在过去几年的支持下，组织培养设施已经处理了 来自EB和对照受试者的大量人类细胞。 它还 维持了大量的自发或病毒转化的 对阐明基因非常有帮助的人类细胞系 表达BMZ大分子，包括VII型胶原蛋白。 这些 已建立的细胞系包括人羊膜内皮细胞系 （WISH）、人表皮样口腔癌细胞系（KB）和人表皮样口腔癌细胞系（WISH）。 纤维肉瘤细胞系（HT-1080），其可商购获得 （ATCC）。

The Tssue Culture Core facilities are of central importance for this Program Project, as they will continue to provide cultured cells from individuals affected with EB, from their unaffected family members, and from unrelated healthy controls for study in all Projects. During the previous years of support, the tissue culture facility has handled a large number of human cells from EB and control subjects. It has also maintained a large number of either spontaneously or virally transormed human cell lines that have been extremely helpful in elucidating gene expression of BMZ macromolecules, including type VII collagen. These established cell lines include a human amniotic endothelial cell line (WISH), a human epidermoid oral carcinoma cell line (KB), and a human fibrosarcoma cell line (HT-1080), which are commercially available (ATCC).