DOUBLE-STRANDED RNA AND THE INTERFERON SYSTEM

本项目的总体目标是了解 通过双链RNA的转录信号。 的表达 几种干扰素诱导基因是由双链 核糖核酸 我们将研究双链RNA是如何调节 基因的转录。 为此，顺式作用序列 将确定双链RNA效应所需的蛋白质， 介导这种诱导的同源反式作用因子将是 分析了 该项目的另一个目的是识别细胞蛋白质 特异性结合双链RNA 这些蛋白质将 纯化后，将分离其cDNA克隆，并将其推定的作用 将评估双链RNA的转录信号传导。 拟议的研究将使人们更好地了解 调节双链RNA诱导基因的表达， 编码许多重要的调节蛋白如干扰素， 白细胞介素和细胞粘附分子。 我们的研究还显示， 使用的信号转导途径之间重叠的性质 干扰素和双链RNA，用于诱导 相同的基因

The overall goal of this project is to understand the mechanisms of transcriptional signaling by double-stranded RNA. the expression of several interferon-inducible genes is directly induced by double-stranded RNA. We will investigate how double-stranded RNA regulates the transcription of genes. For this purpose, the cis-acting sequences required for the effects of double-stranded RNA will be identified and the cognate trans-acting factors which mediate this induction will be analyzed. Another aim of the project is to identify cellular proteins which specifically bind double-stranded RNA. These proteins will be purified, their cDNA clones will be isolated, and their putative roles is transcriptional signaling by double-stranded RNA will be evaluated. the proposed studies will provide a better understanding of the regulation of expression of double-stranded RNA-inducible genes which encode many important regulatory proteins such as interferons, interleukins, and cell adhesion molecules. Our studies also reveal the nature of overlap between the signal transduction pathways used by interferons and double-stranded RNA for inducing transcription of the same genes.