CORE--TUMOR BIOLOGY

这个项目的主要目标是开发新的核酸 杂交技术，基于电荷的先进检测方法 耦合设备，以及自动化样品制备和处理， 在自动化工作站中使用，以分析大的 mRNA种类的数量、杂合性丢失和特定基因 突变的同时，在一个单一的肺组织样本。 是 记录和量化这些设备的性能至关重要， 真实的临床标本。 此外，从这些获得的结果 新开发的设备必须与从 常规方法。 肿瘤生物学核心（TBC）负责 协调样本收集，提供预先表征的模型肿瘤 用于标准化的肿瘤组和未表征肿瘤组 在项目1和项目3中开发的设备。 TBC还将 确定新发现的肺癌遗传变异作为来源 为计划项目增加潜在的有价值的标记。1)到 建立组织库，并跟踪所有相关组织材料 到项目计划。2)准备组织样本， 多参数遗传分析。 3)分析基因变异 在肺癌中使用常规方法来提供多个 参数分析或验证多参数结果 分析. 4)确定其他遗传改变和/或变化 在肺肿瘤发生中潜在重要的mRNA表达中 用于更新程序项目中使用的探头面板。

The main goal of this program project is to develop new nucleic acid hybridization technologies, advanced detection methods based on charge couped devices, and automated sample preparation and handling that could be used in an automated workstation to analyze the expression of large numbers of mRNA species, loss of heterozygosity, and specific gene mutations simultaneously, in a single sample of lung tissue. It is crucial to document and quantify the performance of these devices on real clinical specimens. Additionally, the results obtained from these newly developed devices must be comparable to those obtained from conventional methods. Tumor Biology Core (TBC) is responsible for coordinating sample collection, providing precharacterized model tumor panels and uncharacterized tumor panels for the standardization of devices developed in Project 1 and Project 3. The TBC will also identify newly identified genetic alterations n lung cancers as a source of addition potential valuable markers for the Program Project. 1) To establish a tissue bank and keep track of all tissue materials related to the Program Project. 2) To prepare tissue samples for conventional and multi parameter genetic analysis. 3) To analyze genetic alterations in lung cancers using conventional means to provide standards for multi parameter analysis or validate results from the multi parameter analysis. 4) To identify additional genetic alterations and/or changes in mRNA expression which are potentially important in lung tumorigenesis for up-dating probe panels used in the program project.