INFLUENCE OF EMF ON FREE RADICAL MEDIATED TOXOCOLOGICAL PROCESSES

尽管流行病学研究表明， 暴露于电磁场之间是一种弱关系 (EMF)与癌症目前有几个看似合理的分子 解释这种效应的机制。已知的FREE参与 癌症和其他疾病的病因学中的自由基提高了 电动势可能会以某种方式通过延长寿命来发挥作用 和/或自由基浓度。它已经为许多人所熟知 在有组织的介质(如洗涤剂胶束)中应用的年份 磁场导致平均自由基增加 浓缩和延长自由基的寿命。如果这些 影响发生在体内，那么这可能解释了一些已报道的 电磁场的生物效应。我们的初步研究主要集中在 完整红细胞作为一种测试系统，可以用来研究 MF对自由基过程的影响。我们发现， 光毒性非类固醇抗炎药--酮洛芬 (在结构上与二苯甲酮有关)，导致完全溶血 仅在紫外线照射20分钟后，对人红细胞的影响。 光溶血依赖于氧的存在，并且是 被还原型谷胱甘肽和丁基羟基甲苯抑制 (BHT)提示其机制与脂质过氧化有关。 当在磁场(3500G)中进行紫外线照射时 50%的细胞发生溶血所需的时间(T_)为 显著缩短(78分钟对96分钟)。显著下降 在低至250g的磁场中可以观察到T1/2，但其效应 在100G时消失。我们现在发现，在没有 外加磁场，聚苯乙烯微球 含有磁铁矿(Fe3O4)也显著提高了 光溶血和脂质过氧化。这种影响并不是 当对照微球(即无Fe3O4)或可溶性铁时观察 (FeCl3)的存在这些观察结果表明 与球体相关联的场是导致 溶血率。我们的发现可以归因于一种磁场 田间诱变降低了黄瓜幼虫的系统交叉率 的还原生成的双三线态自由基对 酮洛芬在红细胞膜上的三重态激发 组件。

Although epidemiological studies suggest that there is a weak relationship between exposure to electromagnetic fields (EMF) and cancer there are at present few plausible molecular mechanisms to explain this effect. The known involvement of free radicals in the etiology of cancer and other diseases raises the possibility that EMF may somehow act by increasing the lifetime and/or concentration of free radicals. It has been known for many years that in organized media (e.g. detergent micelles) applied magnetic fields cause an increase in average free radical concentration and lengthen the lifetime of free radicals. If these effects occur in vivo then this may explain some of the reported biological effects of EMF. Our initial studies have focused on the intact erythrocyte as a test system that could be used to study the effects of MF on free radical processes. We have found that ketoprofen, a phototoxic, non-steroidal anti- inflammatory drug (structurally related to benzophenone), caused complete hemolysis of human erythrocytes after only 20 min UV irradiation. Photohemolysis was dependent on the presence of oxygen and was inhibited by reduced glutathione and butylated hydroxytoluene (BHT) suggesting that the mechanism involved lipid peroxidation. When UV irradiation was carried out in a magnetic field (3500G) the time taken for hemolysis to occur in 50% of the cells (t_) was significantly shortened (78 min vs. 96 min). A significant decrease in t1/2 could be observed at fields as low as 250G but the effect disappeared at 100G. We have now found that,in the absence of an applied external magnetic field, polystyrene microspheres containing magnetite (Fe3O4) also dramatically increase the rate of photohemolysis and lipid peroxidation. This effect was not observed when control microspheres (ie no Fe3O4) or soluble iron (FeCl3) were present These observations suggest that the magnetic field associated with the spheres is responsible for the increase in the rate of hemolysis. Our findings can be attributed to a magnetic field induced decrease in the rate of intersystem crossing of the geminate triplet radical pair generated by the reduction of ketoprofen in its triplet excited state by erythrocyte membrane components.