TRANSMISSION ELECTRON MICROSCOPE

此应用程序申请购买Joel JEM-1010变速器的资金 电子显微镜、数字图像采集系统和高分辨率 宾夕法尼亚大学医学中心(UPMC)和 由该研究所支持的电子显微镜核心设备， 糖尿病和内分泌学研究中心(DERC)和癌症中心。 该仪器将取代17年前安装的Joel 100CX显微镜 在用于超微结构研究的核心设备中 50多名由美国国立卫生研究院支持的研究人员发表了200多篇论文。 该应用程序标识了UPMC和NIH支持的17名调查人员 在其他地方作为主要用户组(MUG)。据估计，还会有8- 项目规模较小的每年12名调查人员也将受益 直接从购买这一工具中获得。现有的显微镜 实际使用时间几乎是每周40小时。大量使用导致了 一些机械系统出现可预见和不可预防的恶化。 停机时间增加，变得不可接受，主要是因为 关键系统的更换部件，例如相机机构、旋钮、 等等，必须被制造、重建或从丢弃的东西中回收 乐器。这台新仪器将由一位有经验的人操作 马克杯的专业技术人员或由他们培训的其他人员。 仪器的使用和核心设施的运作是 由研究所的核心研究设施委员会和 由来自马克杯的调查人员和支持组成的咨询委员会 中锋。这种新仪器在电子光学方面取得了进步， 数码相机系统将增强我们提供和 将最先进的超微结构分析传达给 这种杯子的成本比商业公司低。过去的使用情况 本申请中提供的核心设施和文档 证明该工具将使NIH支持的 在这个研究所和其他地方的研究。支持该应用程序 由机构研究核心设施委员会、DERC和癌症 中心。

This application requests funding to purchase a JOEL JEM-1010 transmission electron microscope, digital image acquisition system, and high resolution printer for the University of Pennsylvania Medical Center (UPMC) and the Electron Microscopy Core Facility which is supported by the Institution, the Diabetes and Endocrinology Research Center (DERC) and Cancer Center. The instrument will replace a JOEL 100CX microscope installed 17 years ago in the Core Facility which has been used for ultrastructural studies for over 50 NIH-supported investigators resulting in over 200 publications. This application identifies 17 NIH-supported investigators at UPMC and elsewhere as the Major User Group(MUG). It is estimated an additional 8- 12 investigators per year with less extensive projects also will benefit directly from the purchase of this instrument. The existing microscope is in actual use almost 40 hr per week. The heavy usage has resulted in predictable and unpreventable deterioration of some mechanical systems. Down-time has increased and become unacceptable, primarily because replacement parts for key systems, e.g., the camera mechanism, knobs, etc., have to be fabricated, rebuilt, or scavenged from discarded instruments. The new instrument will be operated by an experienced professional and technical staff for the MUG or others trained by them. Utilization of the instrument and the operational of the Core Facility are controlled by the Institution's Core Research Facilities Committee and an Advisory Committee composed of investigators from the MUG and supporting Centers. The new instrument, with its advancements in electron optics, and the digital camera system will enhance our ability to provide and communicate state-of-the-art ultrastructural analyses to investigators of the MUG at a lower cost than commercial firms. The past utilization of the Core Facility and the documentation provided in this application demonstrate this instrument will significantly benefit NIH-supported research at this Institution and elsewhere. The application is supported by the Institutional Research Core Facilities Committee, DERC, and Cancer Center.