ACQUISTION OF AN ELECTROSPRAY TANDEM MASS SPECTROMETER

尽管在过去的10年中质谱法取得了重大进展， 多年来，这项技术已经扩展到各种各样的生物， 具有无与伦比的灵敏度和特异性的化合物， 科罗拉多大学健康科学中心（UCHSC）是一个主要的 研究机构，没有电喷雾电离（ESI），串联 四极杆（MS-MS）或高分辨率基质辅助激光解吸 仪器在这个校园，没有提供给UCHSC调查人员， 丹佛地区。为了解决这一重大缺陷， 目前NIH资助的几个项目，一组六个主要用户是 申请资金购买多功能ESI MS-MS仪器， 满足当前的分析需求，并有助于提高 UCHSC研究人员意识到质谱法可以解决一个 各种各样的生物化学和生物医学问题。管理部门 UCHSC提供了优良的设施和强大的财政支持， 此次收购旨在确保其高效和长期运营。的 主要使用组由Drs. John A.汤普森，保罗·芬尼西，布拉德 K.查尔斯·本迪亚克麦克亨利，Roy B.作者：Andrew S.卡夫和詹姆斯 L.麦克马纳曼汤普森博士将利用ESI MS-MS研究 细胞毒性酚类的活性代谢物，包括亲电子醌 甲基化物，烷基化蛋白质和DNA，和过氧喹啉，氧化 蛋白质中的蛋氨酸残基。Fennessey博士会用这个仪器 对于正在进行的儿科受试者先天性代谢缺陷的研究， 特别是导致酰基肉毒碱水平升高的疾病。他的团队 也参与蛋白质和肽的分析，这些研究将 由ESI MS-MS大大促进。本迪亚克博士将执行 从人糖蛋白释放的寡糖的结构分析， 和从还原端依次降解的寡糖。 博士麦克亨利将研究DNA的重组亚基蛋白 聚合酶和建立共翻译或翻译后修饰的位点。 他还将通过以下方式确定亚单位之间的相互作用位点： 用交联肽进行研究。琼斯博士和卡夫会利用超感知觉 MS-MS研究复合物的药代动力学和代谢 在生理液体中溶解药物。麦克马纳曼博士将 检测醛氧化酶和黄嘌呤的肽的结构 氧化酶，以确定翻译后修饰的位点， 氧化损伤

In spite of the major advances in mass spectrometry during the past 10 years which have extended this technique to a wide variety of biological compounds with unparalleled sensitivity and specificity, and the fact that the University of Colorado Health Sciences Center (UCHSC) is a major research institution, there are no electrospray ionization (ESI), tandem quadrupole (MS-MS), or high resolution matrix assisted laser desorption instruments on this campus and none available to UCHSC investigators in the Denver area. In order to address this major shortcoming and to support several current NIH-funded projects, a group of six major users are requesting funds to purchase a versatile ESI MS-MS instrument which will fulfill current analytical needs and also serve to enhance the general awareness among UCHSC investigators that mass spectrometry can solve a wide variety of biochemical and biomedical problems. Administrations at UCHSC have provided excellent facilities and strong financial support for this acquisition to assure its efficient and long-term operation. The major use group consists of Drs. John A. Thompson, Paul V. Fennessey, Brad K. Bendiak, Charles S. McHenry, Roy B. Jones, Andrew S. Kraft, and James L. McManaman. Dr. Thompson will utilize the ESI MS-MS to investigate reactive metabolites of cytotoxic phenols, including electrophilic quinone methides which alkylate proteins and DNA, and peroxyquinols which oxidize methionine residues in proteins. Dr. Fennessey will utilize the instrument for ongoing studies on inborn errors in metabolism in pediatric subjects, specifically disorders results in elevated acylcarnitine levels. His group is also involved in protein and peptide analysis and those studies will be greatly facilitated by ESI MS-MS. Dr. Bendiak will be performing structural analyses of oligosaccharides released from human glycoproteins, and oligosaccharides that are sequentially degraded from the reducing end. Dr. McHenry will be examining recombinant subunit proteins of DNA polymerase and establish sites of co-or post-translational modification. He will also identify sites of interaction between subunits through studies with cross-linked peptides. Dr. Jones and Kraft will utilize ESP MS-MS to study the pharmacokinetics and metabolism of complex antineoplastic drugs in physiological fluids. Dr. McManaman will be examining the structures of peptides from aldehyde oxidase and xanthine oxidase to determine sites of post-translational modification and oxidative damage.