DELTAVISION OPTICAL MICROSCOPY WORKSTATION

这项建议要求资金购买基于去卷积的 “DeltaVision”光学显微镜工作站，专为高度 灵敏而精确地获取荧光物质的二维和三维图像 标记的生物标本。我们在BCM的调查人员熟悉 去卷积技术与激光扫描共聚焦显微镜相比的优势 并准备充分利用这一固有的属性 乐器。教职员工专业知识和项目的多样性 包括光学和电子显微镜、分子生物学、发育 生物学、遗传学、细胞生物学和计算生物学。这个 DeltaVision成像中涉及的计算密集型图像处理 的超级计算机资源提供了极好的支持 贝勒生物医学计算和可视化实验室，并将 允许近乎实时处理图像文件。这件乐器将会服务于 BCM多个设施的研究需求及其研究需求 极高的X、Y和Z分辨率数字图像采集 固定组织或细胞，以及分析光学切片的能力 随后进行3D重建。此外，本系统的设计还允许 收集极微弱的信号，能够同时 从最多四个不同的渠道收集荧光， 有机会在以下位置收集相应的高质量相图或DIC图像 每个焦平面。所有这些功能目前都不能用于 贝勒研究人员。除了提供卓越的空间分辨率之外 和敏感性，生物医学研究计划这项新技术 将支持有一个共同的中心主题：集体努力 通过强大的组合来解决现代生物学问题 分子生物学和现代数字显微镜的方法。项目 本申请中描述的内容将能够解决以下问题 生物结构和功能是迄今为止无与伦比的 决议。高度集成的DeltaVision工作站将支持 分子形态的新水平的进展将决定 关键分子在何时何地发挥作用。中包含的软件 这一包将使理解复杂的能力得到增强 酵母细胞水平上的生物结构三维组织 从细胞核到哺乳动物组织。此处请求的仪器将是 关键部件推进贝勒集成显微镜核心设施 并作为德克萨斯医疗中心的共享资源， 包括莱斯大学、休斯顿大学和德克萨斯大学医学院 学校。

This proposal requests funds to purchase a deconvolution-based "DeltaVision" optical microscopy workstation, designed for highly sensitive and precise acquisition of 2- and 3-D images of fluorescently- labeled biological specimens. Our investigators at BCM are conversant with the advantages of deconvolution versus laser scanning confocal microscopy and are well poised to take advantage of the inherent attributes of this instrument. The diversity of expertise and projects among the faculty include optical and electron microscopy, molecular biology, developmental biology, genetics, cell biology and computational biology. The computationally intensive image processing involved in DeltaVision imaging is exceptionally well supported by the super computer resources of Baylor's Biomedical Computation and Visualization Laboratory, and will allow nearly live-processing of image files. This instrument will serve the research needs of multiple facility at BCM whose research demands extremely high X, Y and Z resolution digital image acquisition of live or fixed tissues or cells, and the ability to analyze the optical sections following 3D reconstruction. Moreover, the design of this system allows the collection of extremely faint signals, the ability to simultaneously collect fluorescence from up to four different channels and the opportunity to collect corresponding high-quality phase or DIC images at each focal plane. None of these capabilities are currently available to Baylor researchers. In addition to providing superior spatial resolution and sensitivity, the biomedical research projects that this new technology will support have a central theme in common: a collective effort to approach modern biological questions through the powerful combinatorial approach of molecular biology and modern digital microscopy. Projects described within this application will be able to address questions of biological structure and function at heretofore unapproachable levels of resolution. The high integrated DeltaVision workstation will allow advancement to a new level of molecular morphology that will determine where and when key molecules are functioning. The software included in this package will allow a heightened capability to understand the complex 3-D organization of biological structure from the level of a yeast cell nucleus to mammalian tissues. The instrument requested here will be a pivotal component advancing Baylor's Integrated Microscopy Core facility and serve as a shared resource throughout the Texas Medical Center, including Rice University, The University of Houston and the UT Medical School.