MULTISITE OPTICAL MAPPING SYSTEM

这是一个新的应用程序，以获取多站点光学测绘系统， 在一个科学上强大的和多样化的公共卫生支持的群体中共享 来自华盛顿大学6个不同部门的调查 医学院（内科心胸外科，病理学， 细胞生物学和生理学，分子生物学和药理学， 实验神经病学和神经外科学）。光学标测 技术在华盛顿大学是不存在的。因此，委员会认为， 购买这种仪器将对 用户组当前和未来活动的进展情况。拟议 光学测绘的应用集中在3个主要领域： 1)正常心脏病分子决定因素的阐明 电生理学和描绘的机制， 小鼠模型，其中编码特定离子的基因的表达 通道和其他蛋白质是基因操纵; 2）识别 房性和室性心律失常的机制和表征 通过综合电生理学分析， 人类疾病的动物模型和从心脏病获得的组织中 患者;和3）分析潜在的发展机制， 中枢神经系统的反应可塑性和 局部神经元活动和局部脑血流。请求的 仪器将安置在中心的一个中央核心设施中 在华盛顿大学医学院的心血管研究。 用户组的组成旨在促进科学 基础科学家和临床研究者之间的合作， 鼓励和增强心脏电生理学之间的相互作用， 以及华盛顿大学的神经生物学社区。本技术 资源也将是至关重要的，在我们努力招募新的研究 分子电生理学系。虽然没有多站点 光学测绘系统在华盛顿大学，成员的用户 小组与被公认为世界领先的实验室合作， 光学测绘应用他们还拥有丰富的经验， 常规（基于电极）电生理标测， 相关光学方法和计算机系统在采集中的应用 分析复杂的数据集。光学测绘的建立 资源将加强当前和未来的研究计划和科学 华盛顿大学研究人员的贡献。

This is a new application to acquire a Multisite Optical Mapping System to be shared among a scientifically strong and diverse group of PHS-supported investigations from 6 different departments at Washington University School of Medicine (Internal Medicine Cardiothoracic Surgery, Pathology, Cell Biology and Physiology, Molecular Biology and Pharmacology, Experimental Neurology and Neurological Surgery). Optical mapping technology does not exist at Washington University. Accordingly, acquisition of this instrumentation will have a major impact on the progress of current and future activities of the user group. The proposed applications of optical mapping are focused on 3 major areas of interest: 1) elucidation of molecular determinants of normal cardiac electrophysiology and delineation of mechanisms of arrhythmogenesis in mouse models in which the expression of genes encoding specific ion channels and other proteins is genetically manipulated; 2) identification of mechanisms of atrial and ventricular arrhythmias and characterization of novel therapies by integrated electrophysiological analysis in large animal models of human disease and in tissues obtained from heart disease patients; and 3) analysis of mechanisms underlying development and reactive plasticity of the central nervous system and mechanisms linking local neuronal activity and local cerebral blood flow. The requested instrumentation will be housed in a central core facility in the Center for Cardiovascular Research at Washington University School of Medicine. The composition of the user group is designed to facilitate scientific collaborations between basic scientists and clinical investigators and to encourage and enhance interactions between the cardiac electrophysiology and neurobiology communities at Washington University. This technical resource will also be crucial in our efforts to recruit new research faculty in molecular electrophysiology. Although there are no multisite optical mapping systems at Washington University, members of the user group have collaborated with laboratories recognized as world leaders in optical mapping application. They also have extensive experience in conventional (electrode-based) electrophysiological mapping in the use of related optical methods and computer systems applications in acquisition and analysis of complex data sets. The establishment of an optical mapping resource will enhance current and future research programs and scientific contributions by investigators at Washington University.