DELTA VISION DECONVOLUTION FLUORESCENCE MICROSCOPE

DeltaVision光学切片(去卷积)显微镜系统及SGI 工作站是由四个主要用户和一个社区组成的小组请求的 加州大学伯克利分校的未成年用户。目前， 加州大学伯克利分校没有这样的乐器。项目包括 减数分裂、视网膜变性、 胃壁细胞分泌、染色质相关蛋白与性别 染色体失活。所有用户目前都有NIH的资金。其中两个 调查人员对这种仪器和另一种仪器有丰富的经验 两名研究人员在他们的研究中使用了反卷积显微镜。 图像被记录在冷却的CCD(电荷耦合设备)相机上，该相机 具有高分辨率和低光敏感度。滤器 组合，显微镜工作台(X、Y和Z)的电动控制 方向)、灯和ccd快门以及ccd数据采集都是 计算机控制并耦合到硅图形显示系统。这个 DeltaVision系统是任何低亮度成像的理想选择 危急时刻。这在观察活细胞方面非常重要，因为很久以前 长期暴露在强光下会漂白染料并损害 样本。当一个样本被多个 探头，其中一些很容易漂白。三维 反卷积程序将提供高分辨率的三维图像 与使用共聚焦显微镜获得的结果相当或更好。一个 用于图像分析和体绘制的软件包，包括 立体视觉是该系统不可或缺的一部分，而且更有用 而不是随共焦显微镜一起出售的图像分析包。这个 DeltaVision系统使用具有连续发射的UV光源 允许监测多个荧光探针的光谱 同时。显微镜将被放置在 分子和细胞生物学系生命科学增建楼， 加州大学伯克利分校。乐器将由一位训练有素的人监督。 职员研究助理。

A DeltaVision Optical Sectioning (deconvolution) microscope system and SGI workstation are requested by a group of four major users and a community of minor users at the University of California, Berkeley. Currently, there is no instrument of this kind at UC Berkeley. Projects include studies of chromosome arrangement in meiosis, retinal degeneration, secretion in gastric parietal cells, chromatin associated proteins and sex chromosome inactivation. All users have current NIH funding. Two of the investigators have extensive experience with this instrument and the other two investigators have used deconvolution microscopy in their research. Images are recorded on a cooled CCD (charged-coupled device) camera that has both high resolution and low light level sensitivity. Filter combinations, motorized control of the microscope stage (X, Y & Z directions), lamp and CCD Shutters, and CCD data collection are all computer controlled and coupled to a Silicon Graphics display system. The DeltaVision system is ideal for any imaging where low light levels are critical. This is very important in looking at living cells since long term exposure to high light intensities bleach dyes and damage the specimen. It is also useful when a specimen is stained with multiple probes, and some of them are easily bleached. Three dimensional deconvolution programs will give high resolution three dimensional images equivalent or better than that achieved using confocal microscopy. A software package for image analysis and volume rendering, including stereographic viewing is integral to the system and is much more useful than the image analysis packages sold with confocal microscopes. The DeltaVision system uses a UV light source with a continuous emission spectrum permitting several fluorescent probes to be monitored simultaneously. The microscope will be placed at a shared use site in the Life Sciences Addition building, Department of Molecular and Cell Biology, UC Berkeley. The instrument will be under the supervision of a trained Staff Research Associate.