CORE--CELL ISOLATION AND CULTURE

细胞分离和培养核心提供专业知识和服务 所有实验的设计、执行和解释 使用培养的细胞和细胞系。核心的人员已经 在细胞培养领域的经验。《公约》的具体目标 核心是1)分离和培养原代细胞和细胞系所需的 由本申请书中提议的五个项目的每个调查员， 2)保持特殊的细胞系(例如，稳定转染的气管 心肌细胞)用于拟议的研究，3)验证质量 对建议中使用的所有细胞培养和细胞系进行控制 调查，4)维护这些所需的设施和设备 项目，以及5)根据需要开发细胞培养的新技术 每个项目，并适当地指导新的项目研究人员 技术。该核心将提供的具体服务包括： 特异性原代细胞和细胞的分离、生长和制备 线路，关于试验设计、提供和维护的咨询 专门化细胞系，使用所提供的质粒对细胞系进行转基因 由具体项目中的调查人员提供所需数据 验证细胞系和转基因细胞。设施和 参与这一核心的人员在每一项科学 项目。每个项目都需要隔离和/或培养 用于实验的细胞。有一些常见的技术将具有 更有效率的规模经济。此外，常见的设备需求 对于细胞的长期储存，以及细胞的转染性，可以满足更多 通过核心设施轻松实现。最后，我们有必要的 可用于应对和适应新方向的经验和资源 调查的结果。所有项目都将从与此核心的互动中受益 设施。

The Cell Isolation and Culture Core provides expertise and services in the design, performance and interpretation of all experiments in which cultured cells and cell lines are used. Personnel in the core have experience spanning the field of cell culture. The specific aims of the core are to 1) isolate and culture primary cells and cell lines required by each investigator for the five projects proposed in this application, 2) maintain special cell lines (e.g., stably-transfected tracheal myocytes) required for proposed investigations, 3) validate quality control for all cell cultures and cell lines used in proposed investigations, 4) maintain facilities and equipment required for these projects, and 5) develop new techniques in cell culture as required for each project, and instruct new project research personnel in proper technique. The specific services to be provided by this core are: isolation, growth and preparation of specific primary cells and cell lines, consultation on experimental design, provision and maintenance of specialized cell lines, transfection of cell lines using plasmids provided by investigators in the specific projects, and provision of data required to validate cell lines and transfected cells. The facilities and personnel involved in this core have a role in each of the scientific projects. Each project shares a need for isolation and/or culture of cells for use in experiments. There are common techniques that will have a more efficient economy of scale. In addition, common equipment needs for long-term storage of cells, and cell transfections, can be met more easily through the Core Facility. Finally, we have the necessary experience and resources available to respond and adapt to new directions of inquiry. All projects will benefit from the interaction with this Core Facility.