CORE--FLOW CYTOMETRY CELL SEPARATION FACILITY

流式细胞术在正常造血干细胞纯化中的应用 祖细胞和干细胞以及分析表达和 细胞表面和细胞内分子的功能， 造血细胞是这项赠款项目的核心。流 细胞计量术可用于基于细胞的形态学特征分析和纯化活细胞。 细胞表面决定因素的表达、物理性质（细胞大小 通过光散射测定），并用活体染料标记。流 流式细胞术也可用于研究细胞的同时表达， 表面决定簇和细胞内分子，包括蛋白质， mRNA以及DNA含量用于细胞周期分析。流式细胞术 Core将进行所有细胞分析和细胞分选， 单细胞沉积（克隆），使用流式细胞术。 流式细胞仪将用于每个项目在这个赠款， 纯化祖细胞和干细胞，用于直接的体外和体内研究 并研究细胞表面和细胞内分子的表达， 干细胞及其后代。因此，流式细胞术的功能 核心设施将进行多参数流式细胞术 分析细胞系和培养并感染的原代细胞， 多参数细胞分选和单细胞克隆，以分离 人和狒狒CD34+骨髓和血细胞以及鼠 造血干细胞是体外和体内研究所必需的。

The use of flow cytometry for the purification of normal hematopoietic progenitor and stem cells as well as to analyze the expression and function of defined cell surface and intracellular molecules expressed by hematopoietic cells is central to the projects in this grant. Flow cytometry can be used to analyze and purify viable cells based on the expression of cell surface determinants, physical properties (cell size determined by light scattering), and labeling with vital dyes. Flow cytometry also can be used to study the simultaneous expression of cell surface determinants and intracellular molecules including proteins and mRNA, as well as DNA content for cell cycle analysis. The Flow Cytometry Core will perform all cell analysis and cell sorting, for both bulk and single cell deposition (cloning), using flow cytometry. Flow cytometry will be used by each of the projects in this grant to purify progenitor and stem cells for direct in vitro and in vivo studies and to study expression of cell surface and intracellular molecules by stem cells and their progeny. Therefore the function of the flow cytometry core facility will be to perform multiple parameter flow cytometric analysis of cell lines and cultured and infected primary cells and multiparameter cell sorting and single cell cloning to isolate subsets of human and baboon CD34+ marrow and blood cells as well as murine hematopoietic stem cells necessary for in vitro and in vivo studies.