Molecular Diagnosis of Feline Infectious Peritonitis vs Enteric Corona Virus

已经开发了一种使用RT-PCR的方案 (逆转录聚合酶链式反应)与巢式 检测猫科动物感染性腹膜炎病毒的合成寡聚体 RNA及其与猫肠道冠状病毒的鉴别 (FECV)RNA。因为人们认为FECV的一个突变是 FIP病毒的来源，这项技术对于指示 仅存在最致命的FIP病毒。它还可以给 兽医潜在的FIP暴发的迹象和方法 为消除美国国立卫生研究院所依赖的猫咪中的病毒 调查人员。许多样品已经用这一方法进行了处理 协助美国国立卫生研究院设施根除这种病原体的议定书 以评估这种方法学在流行病学研究中的有效性 学习。

A protocol has been developed using RT-PCR (reverse transcriptase polymerase chain reaction) with nested synthetic oligomers to detect Feline Infectious Peritonitis (FIP) viral RNA and to distinguish it from Feline Enteric Corona Virus (FECV) RNA. Since it is thought that a mutation in FECV is the source of the FIP virus, this technique is important to indicate the presence of the mostly fatal FIP virus alone. It can also give the veterinarian an indication of a potential FIP outbreak and a method for eliminating the virus from catteries relied on by NIH investigators. Many samples have been processed using this protocol to assist NIH facilities in eradication of this pathogen and for evaluating the efficacy of this methodology for epidemiological studies.