EXPERIMENTAL LEPROSY & VACCINE STUDIES IN MONKEYS: MYCOBACTERIA

我们分析和评估了收集的纵向免疫学数据 接种后3-4年(使用活卡介苗或卡介苗+) 热灭活麻风分枝杆菌(HKML)、增强后(PB)和 挑战后(PC)与现场ML。恒河猴(Rm)少杆菌 易(PB)的，作为一个物种]在临床上受到显著保护 卡介苗或卡介苗+HKML；煤烟芒果猴(SMM)[多菌型 (MB)-倾向于作为一个物种]仅受卡介苗的部分保护，但 临床上对卡介苗+HKML易感。一致 临床结果和胚泡反应之间有相似之处。 外周血单个核细胞对ML抗原和结核菌素血清的反应 抗酚糖脂-I(PGL-I)抗体亚型(Ig G和Ig M) 反应、血清PGL-I抗原水平、血淋巴细胞亚群 携带CD4、CD8、CD16、CD20、CD4CD29和CD4CD45表型标志物 以及乐普宁皮试结果。有多个复合体， 免疫应答中相互关联的剂量依赖关系 确定麻风病易感性/耐药性的ML抗原。这个 机制可能涉及RM和SMM之间的细微差异 细胞因子的产生和/或反应，这些反应在 具有PB和MB易感性的个体。研究正在进行中 应用聚合酶链式反应检测比较细胞因子图谱的研究进展 在这项研究中，从动物的纵向获得的病变活检 帮助

We analyzed and evaluated longitudinal immunologic data gathered over a 3-4 year period post vaccination (PV) (with live BCG or BCG + heat-killed Mycobacterium leprae, HKML), post-boosting (PB) and post-challenge (PC) with live ML. Rhesus monkeys (RM) [paucibacillary (PB)-prone, as a species] were significantly protected clinically by BCG or BCG + HKML; sooty mangabey monkeys (SMM) [multibacillary (MB)-prone, as a species] were partially protected by BCG alone, but were rendered clinically more susceptible by BCG + HKML. Consistent parallels were seen between clinical results and blastogenic responses of blood mononuclear cells to ML antigens and to tuberculin, serum anti-phenolic glycolipid-I (PGL-I) antibody isotype (IgG & IgM) responses, serum PGL-I antigen levels, blood lymphocyte subsets carrying CD4, CD8, CD16, CD20, CD4CD29 and CD4CD45 phenotypic markers and lepromin skin test results. There are multiple complex, interrelated dose-dependent relationships in immunologic responses to ML antigens that determine leprosy susceptibility/resistance. The mechanisms may involve subtle differences between RM and SMM in cytokine production and/or responses, which model responses in individual humans with PB vs MB predispositions. Studies are in progress to determine comparative cytokine profiles by PCR in longitudinally-obtained lesion biopsies from animals in this study to help