BINDING OF COPPER(II) TO PULMONARY ENDOTHELIAL CELLS

采用电子自旋共振(ESR)方法检测肺动脉铜离子。 内皮细胞在明胶包裹的Biosilon微珠上生长融合 (请参见高亮显示)。在这些材料中加入150微摩尔的铜(II) 细胞，从细胞和培养上清液中获得EPR谱 不同时间与铜(II)的孵化以及在 不存在铜(I)的比色剂--铜铜试剂。一份EPR G(平行)=2.18的第二类铜(II)位的信号 A(平行)=180G归因于与细胞结合的铜离子。添加 它不仅与铜(I)形成络合物，而且与铜(I)形成络合物。 Cu(II)，g(平行)=2.19，A(平行)=160g。其他研究 计划确定与铜(II)结合的氮素的数量 对细胞采用低频、S波段、电子顺磁共振等方法。进一步的工作是 计划帮助理解本网站的重要意义 到跨膜电子传输。

ESR methods were used to detect cupric ion on pulmonary arterial endothelial cells grown to confluence on gelatin-coated Biosilon beads (see highlight). Upon addition of 150 micro molar Cu(II) to these cells, EPR spectra were obtained from cells and supernate after various times of incubation with Cu(II) and in the presence and absence of bathocuprione, a colorimetric agent for Cu(I). An EPR signal for a type 2 Cu(II) site with g(parallel)=2.18 and A(parallel)=180G is attributed to cupric ion bound to cells. Addition of bathocuprione not only formed a complex with Cu(I), but also with Cu(II) with g(parallel)=2.19 and A(parallel)=160G. Additional studies are planned to determine the number of nitrogens bound to the Cu(II) on cells using low frequency, S-band, EPR methods. Further work is planned to help understand the significance of this site with respect to trans-membrane electron transport.