REGULATION OF CYTIDYLYLTRANSFERASE ACTIVITY

该实验室的工作重点是哺乳动物磷脂酰胆碱代谢的调节，这是由胆碱激酶和CTP：胆碱磷酸胞基转移酶（CCT）调节的。我们假设细胞胆碱激酶活性是由胆碱激酶同工酶在某些细胞中的选择性表达和对某些信号的响应来调节的。我们从哺乳动物和线虫中分离、表达和分析胆碱激酶同种异构体的表达模式。此外，我们正在研究磷酸化和脂质对CCT活性的调节，并一直在研究该酶的催化机制。我们使用重组DNA技术进行所有这些研究，并需要使用GCG数据库和序列分析软件来继续进行研究。

Work in this laboratory has focused on the regulation of mammalian phosphatidylcholine metabolism, which is regulated by the enzymes choline kinase and CTP:cholinephosphate cytidylyltransferase (CCT). We hypothesize that cellular choline kinase activity is modulated by the selective expression of choline kinase isozymes in certain cells and in response to certain signals. We are isolating, expressing, and analyzing the expression patterns of choline kinase isoforms from mammals and the nematode, C. elegans. In addition, we are studying the regulation of CCT activity by phosphorylation and lipids, and have been studying the catalytic mechanism of this enzyme. We use recombinant DNA techniques for all these studies, and require the use of the GCG data base and sequence analysis software to continue them.