BOWEL FLORA ROLE IN CONVERSION OF GLUCOSINOLATES TO ISOTHIOCYANATES

确定肠道植物群是否有助于硫苷 在人体代谢中，我们评估了标准术前 肠道准备对二硫代氨基甲酸盐排泄的影响 成熟花椰菜中的芥子油苷。 为了制备硫代葡萄糖苷，小花 从冰包装的西兰花被切断，并立即在蒸汽煮， 夹套釜（8L水，5分钟）。 小花被移走，剩下的 将液体减少到其原始体积的约一半（超过5分钟）， 将该混合物均质化， 在一个Waring Blendor。 取样品进行分析， 储存在-80 ℃。 个体剂量包括112 g鲜重 西兰花在182毫升，含有100摩尔非吲哚芥子油苷，0.5 mol异硫氰酸酯和无残余黑芥子酶活性;萝卜硫苷 占硫代葡萄糖苷的76.1%。

To determine whether enteric flora contribute to glucosinolate metabolism in humans, we evaluated the effect of a standard preoperative bowel preparation on dithiocarbamate excretion after ingestion of glucosinolates from mature broccoli. To prepare glucosinolates, florets from ice-packed broccoli were cut and immediately boiled in a steam- jacketed kettle (8 L water, 5 min). Florets were removed, the remaining liquid was reduced to about half its original volume (over 5 min) and was added back to the cooked florets, and the mixture was homogenized in a Waring Blendor. Samples were taken for analysis and the homogenate was stored at -80 C. Individual doses consisted if 112 g fresh weight broccoli in 182 mL, containing 100 mol non-indole glucosinolates, 0.5 mol isothiocyanates and no residual myrosinase activity; glucoraphanin constituted 76.1% of the glucosinolates.