INTRASUBJECT VARIABILITY IN METABOLISM OF GLUCOSINOLATES (HS33A)

确定肠道菌群是否对硫代葡萄糖苷有贡献 在人类的新陈代谢中，我们评估了标准的术前效果 肠道准备对摄入二硫代氨基甲酸酯排泄的影响 成熟西兰花中的硫代葡萄糖苷。准备硫代葡萄糖苷、小花 从冰包装的西兰花切下来，立即在蒸气中煮熟- 夹套水壶(8 L水，5分钟)。小花被移走了，剩下的 液体减少到原来体积的一半左右(超过5分钟) 加回煮熟的小花中，混合物被均质 在瓦林的搅拌机里。取样本进行分析，并将匀浆 储存在-80摄氏度。个人剂量包括112克鲜重 182毫升西兰花，含100摩尔非吲哚硫代葡萄糖苷，0.5 摩尔异硫氰酸酯和无残留芥子酶活性；葡萄糖萝卜素 占硫代葡萄糖苷的76.1%。

To determine whether enteric flora contribute to glucosinolate metabolism in humans, we evaluated the effect of a standard preoperative bowel preparation on dithiocarbamate excretion after ingestion of glucosinolates from mature broccoli. To prepare glucosinolates, florets from ice-packed broccoli were cut and immediately boiled in a steam- jacketed kettle (8 L water, 5 min). Florets were removed, the remaining liquid was reduced to about half its original volume (over 5 min) and was added back to the cooked florets, and the mixture was homogenized in a Waring Blendor. Samples were taken for analysis and the homogenate was stored at -80 C. Individual doses consisted if 112 g fresh weight broccoli in 182 mL, containing 100 mol non-indole glucosinolates, 0.5 mol isothiocyanates and no residual myrosinase activity; glucoraphanin constituted 76.1% of the glucosinolates.