CONVERSION OF GLUCOSINOLATES TO ISOTHIOCYNATES IN SUBJECTS FED BROCCOLI SPROUTS

4名男性志愿者(43岁至53岁；3名黑人，1名白人) 登记、给药和监测。在最初的三天洗礼中 期间，二硫代氨基甲酸盐的总排泄量从无法检测到到70.7 摩尔。然而，对于所有四名志愿者来说，水平都低于 检测(0.124摩尔/8小时)至少比第一次检测早16小时 西兰花发芽剂量。在所有四个科目和所有三个科目中 准备，尿二硫代氨基甲酸盐排泄量在前四个月达到峰值 除一名受试者外，所有受试者在硫代葡萄糖苷排泄后均为数小时 在4-8小时的采集过程中达到顶峰。尽管西兰花 萌芽硫代葡萄糖苷和异硫氰酸酯的剂量相等(100摩尔)， 异氰酸酯制剂的尿量几乎是对照组的7倍 二硫代氨基甲酸酯(89.6 1 5.88摩尔，范围83.9-95.9)比 硫代葡萄糖苷制剂(13.1 1 1.65摩尔，范围11.3-15.7)。这 差异有统计学意义(p=0.0003，双尾配对 T检验)。虽然黛康后总二硫代氨基甲酸盐排泄量 制剂显然超过了剂量的2.9倍，其 对西兰花异硫氰酸酯结果的贡献，占总数的3% 排泄物，仍然可以忽略不计。

Four male volunteers (43 to 53 years old; 3 black, 1 white) were enrolled, dosed, and monitored. During the initial three day washout period, total dithiocarbamate excretion ranged from undetectable to 70.7 mol. However, for all four volunteers, levels fell below the limit of detection (0.124 mol/8 hour) by at least 16 hrs prior to the first broccoli sprout dose. In all four subjects, and for all three preparations, urinary dithiocarbamate excretion peaked in the first four hours for all but one subject, whose excretion after glucosinolates peaked in the 4-8 hour collection. Despite the fact that the broccoli sprout glucosinolates and isothiocyanates doses were equal (100 mol), the isiocyanate preparation yielded nearly seven times more urinary dithicarbamates (89.6 1 5.88 mol, range 83.9 - 95.9) than did the glucosinolate preparation (13.1 1 1.65 mol, range 11.3 - 15.7). This difference is statistically significant (p = 0.0003, two-tailed paired t-test). Although total dithiocarbamate excretion after the daikon preparation apparently exceeded the dose by a factor of up to 2.9, its contribution to the broccoli isothiocyanate results, at 3% of total excretion, remain negligible.