LASER MICROBEAM MANIPULATION OF CELL MORPHOGENESIS IN FUGAL HYHAE: CELL GROWTH

这个项目是整个研究计划的关键部分 目的是为了准确理解 真菌菌丝中细胞生长的过程。 到目前为止，唯一的技术， 实验证明，适合于本地和无损 操纵生长的真菌的大小、速度、方向和形状， 菌丝是使用红外（820 nm）激光微束定位， 移动细胞器复合体和单个细胞器， 细胞生长 这些细胞成分构成细胞膜和细胞壁 扩大菌丝顶端表面的材料， 细胞的局部区域和沿着梯度， 菌丝端部呈现一个特征形状。 我们可以控制 通过移动和重新定位， 通过捕获或“追逐”细胞器，用激光照射它们， 它们被传送到小区中的预定位置。

This project is a critical part of an overall research program aimed at developing a precise understanding of the detailed mechanism of cell growth in fungal hyphae. To date, the only technique that has proven suitable for experimentally, locally, and nondestructively manipulating the size, rate, direction, and shape of growing fungal hyphae is the use of infared (820 nm) laser microbeams to position and move the organellar complex and individual organelles which mediate cell growth. These cell components contribute membrane and cell wall materials to expand the surface of the hyphal tips in precise localized regions of the cell and along a gradient which causes a hyphal tip to assume a characteristic shape. We can control the subtleties of cell morphogenesis at will by moving and repositioning organelles either by trapping or "chasing", them with a laser so that they are transported to predetermined sites in the cell.