POSITIONING FOR KAPPA GONIOMETER & AN XRAY DETECTOR FOR PROTEIN CRYSTALLOGRAPHY

一种坚固的高精度支撑和定位平台， Huber单晶3轴Kappa几何测角仪和 X射线区域探测器（设计用于容纳ADSC Quantum-4 MAR 345成像板检测器的CCD检测器），用于蛋白质 1998年完成了对BL 9 -2的晶体学实验。 这 载物台安装在现有的桌子上，该桌子具有垂直，水平， 俯仰和偏航调整。 支持阶段提供独立的 测角仪和探测器的大规模平移 沿着X射线束方向。 该检测器还具有 在水平和垂直方向上进行大规模平移调整， 垂直方向和水平旋转轴， 探测器可以在任何垂直方向指向样品晶体 位置 每个动作都是电动的，编码器提供 位置读数为15米。 在第一个系统之后， 经过测试和提炼，它将在每一种蛋白质上复制， 晶体学光束线 这个定位阶段的组合 和卡帕几何测角器，将有可能收集 在任何一般样本中， 导向 该设备项目由DOE/OBER提供资金， 这是国家卫生研究院拨款的工资支持。

A sturdy and high precision support and positioning stage, for both a Huber single-crystal 3-axis Kappa-geometry goniometer and an x-ray area detector (designed to accommodate either a ADSC Quantum-4 CCD detector of a MAR345 imaging plate detector), for protein crystallographic experiments on BL9-2 was completed in 1998. This stage was mounted on an existing table which has vertical, horizontal, pitch and yaw adjustments. The support stage provides independent large scale translations for both the goniometer and the detector along the x-ray beam direction. The detector is also provided with large scale translational adjustments in both the horizontal and vertical directions, and a horizontal rotation axis so that the detector can be pointed towards the sample crystal at any vertical position. Each motion has been motorized, with encoders providing position readout of 1 5m. After this first system has been further tested and refined, it will be replicated on each of the protein crystallography beam lines. The combination of this positioning stage and the Kappa-geometry goniometer, will make it possible to collect data from a large volume of reciprocal space in any general sample orientation. This equipment project is funded by DOE/OBER, with some salary support from this NIH grant.