XRAY CRYSTALLOGRAPHY OF PEPTIDE RNA & OLIGOPEPTIDES IMPLICATED IN DIABETES

多肽-RNA复合体蛋白质-RNA识别过程是 对理解其在大分子中的生物学作用的重要性 集合。蛋白质结合往往伴随着大规模的 RNA结构重排。诱导的RNA结构表现出 与变化相关的非标准碱基配对和堆积 凹槽的尺寸。为了更好地理解 RNA的结构曲目，我们试图结晶一种 含有富含嘌呤五环的15碱基非标准RNA发夹 在含有21个氨基酸的Lambda N抗终止肽的复合体中 酸残留物(1)。最近这个复合体的非常小的晶体是 获得。亮氨酸拉链多肽：CHOP(C/EBP同源蛋白) 是C/EBP(C-末端增强子结合蛋白)家族的成员。 CHOP被认为参与调节细胞生长。 压力很大的环境。CHOP在细胞中以二聚体的形式与其他 C/EBP家族的成员，具有强烈的异二聚化 优先于均二聚反应(2)。我们用的是一种30的排骨肽 到36个残基，其中几个亮氨酸表示亮氨酸 拉链。然而，该序列与已知结构不同 亮氨酸拉链图案。MODY基因产物的推定亮氨酸拉链 HNF-1α：我们已经获得了MODY野生型寡肽的晶体 片段(33个残基)。几种突变体的结晶试验 与导致儿童糖尿病有关的基因 出去。我们计划使用以下工具进行结构确定 同步辐射，因为获得了相当完整和高的 在实验室x射线源上很难获得分辨率数据，因为它很小 水晶。因为我们不能免费使用家庭电源设备 此外，我们无法获得任何合理的数据集。我们完全依赖于 关于你的分配来解决上面的结构与非常 在结构生物学中的重要应用。

Peptide-RNA ComplexThe protein-RNA recognition process is important in understanding its biological role in macromolecular assembly. Protein binding is often accompanied by large-scale rearrangement of RNA structure. Induced RNA structures exhibit non-standard base pairing and stacking in association with changes in the dimension of grooves. In order to understand the diverse structural repertoire of RNA, we have attempted to crystallize a 15-base non-standard RNA hairpin containing a purine-rich penta-loop in complex with a Lambda N antitermination peptide containing 21 amino acid residues(1). Recently very small crystals of this complex are obtained. Leucine Zipper Peptides: CHOP (C/EBP homologous protein) is a member of the C/EBP (C-terminal enhancer binding protein) family. CHOP is thought to participate in regulating cell growth under stressful conditions. CHOP is found in cells as a dimer with other members of the C/EBP family, with hetero-dimerization being strongly preferred to homodimerization(2). We have used a CHOP peptide of 30 to 36 residues in length with several Leucines indicative of a leucine zipper. However, the sequence is different from the known structures of leucine-zipper motif. Putative Leucine Zipper of MODY Gene Product HNF-1Alpha: We have obtained crystals of MODY wild type oligopeptide fragment (33 residues). Crystallization trials with several mutants which are implied in causing diabetes in children are being carried out. We plan to carry out structure determination with the use of synchrotron radiation, since obtaining reasonably complete and high resolution data is difficult on a laboratory x-ray source with small crystals. Since we do not have free access to home source equipment also, we are unable to get any reasonable data set. We solely depend on on your allocation to solve the above structures with very important applications in Structural Biology.