CRYSTAL STRUCTURE OF RAB GERANYLGERANYL TRANSFERASE

Rab香叶基香叶基转移酶（GGT）催化以下反应， 将20个碳的香叶基香叶基连接到C-末端半胱氨酸上 小Ras样GTP酶家族的基序，Rab蛋白。 Rab GGT包括两个紧密相关的？65 kD和38 kD亚基 kD，分别。 我们在P1中获得了单个GGT晶体 空间群，在我们实验室中分辨率可达2.5E。 所以 到目前为止，我们已经确定了两个重原子衍生物，但由于 由于P1空间群的限制，我们无法收集到 在我们的实验室中，这些衍生物的完整异常数据集。 我们相信同步加速器上的可调谐X射线源和快速数据 收集与CCD探测器将使我们能够利用 来自这些导数的异常信号用于定相，并导致 最终解决GGT结构。

Rab geranylgeranyl transferase (GGT) catalyzes the reaction that attaches the 20 carbon geranylgeranyl group to the C-terminal cystein motifs of a family of small Ras-like GTPase, the Rab proteins. Rab GGT comprises two tightly associated ? and _ subunits of 65 kD and 38 kD, respectively. We have obtained single GGT crystals in the P1 space group which diffract to 2.5 E resolution in our laboratory. So far we have identified two heavy atom derivatives, but due to the limitation of the P1 space group, we were unable to collect the complete anomalous data set on these derivatives in our laboratory. We believe that tunable X-ray source at synchrotron and rapid data collection with the CCD detector will enable us to make use of the anomalous signals from these derivatives for phasing, and lead to the final solution of the GGT structure.